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Literature summary for 1.4.3.2 extracted from

  • Mandal, S.; Bhattacharyya, D.
    Two L-amino acid oxidase isoenzymes from Russells viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs (2008), FEBS J., 275, 2078-2095.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-aminobenzoic acid three and two binding sites in isoforms L1 and L2, respectively Daboia russelii russelii
additional information initial uncompetitive inhibition of L1 followed by mixed inhibition at higher concentrations suggesting the existence of two different inhibitor-binding sites distinct from the substrate-binding site. In the case of L2, initial linear competitive inhibition followed by mixed inhibition suggesting the existence of two nonoverlapping inhibitor-binding sites, one of which is the substrate-binding site Daboia russelii russelii
N-acetyl-L-tryptophan amide inhibition of both isoforms L1 and L2 Daboia russelii russelii
N-acetyltryptophan inhibition of both isoforms L1 and L2 Daboia russelii russelii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00144
-
L-isoleucine isoform L1 Daboia russelii russelii
0.00189
-
L-isoleucine isoform L2 Daboia russelii russelii
0.0493
-
L-phenylalanine isoform L2 Daboia russelii russelii
0.052
-
L-tyrosine isoform L1 Daboia russelii russelii
0.0665
-
L-phenylalanine isoform L1 Daboia russelii russelii
0.211
-
L-tryptophan isoform L1 Daboia russelii russelii
0.223
-
L-methionine isoform L2 Daboia russelii russelii
0.235
-
L-tryptophan isoform L2 Daboia russelii russelii
0.297
-
L-methionine isoform L1 Daboia russelii russelii
0.538
-
L-tyrosine isoform L2 Daboia russelii russelii
0.6
-
L-leucine isoform L2 Daboia russelii russelii
0.75
-
L-leucine isoform L1 Daboia russelii russelii

Organism

Organism UniProt Comment Textmining
Daboia russelii russelii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration followed by ion exchange chromatography, to homogeneity Daboia russelii russelii

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Daboia russelii russelii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.94
-
isoform L2 Daboia russelii russelii
8.96
-
isoform L1 Daboia russelii russelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + H2O + O2
-
Daboia russelii russelii 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-leucine + H2O + O2
-
Daboia russelii russelii 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2
-
Daboia russelii russelii 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2 maximum catalytic efficiency for both isoforms Daboia russelii russelii phenylpyruvate + NH3 + H2O2
-
?
L-tryptophan + H2O + O2
-
Daboia russelii russelii 3-indole-2-oxopropanoate + NH3 + H2O2
-
?
L-tyrosine + H2O + O2
-
Daboia russelii russelii p-hydroxyphenylpyruvate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000-63000, purified isoforms L1 and L2, size exclusion HPLC and SDS-PAGE Daboia russelii russelii

Synonyms

Synonyms Comment Organism
LAAO
-
Daboia russelii russelii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
both isoforms are stable up to 50°C but are sharply inactivated above 60°C Daboia russelii russelii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.8 both isoforms retain 100% activity, drastic inactivation above and below this range Daboia russelii russelii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Daboia russelii russelii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01189
-
2-aminobenzoic acid isoform L2, free enzyme Daboia russelii russelii
0.02544
-
2-aminobenzoic acid isoform L2, enzyme-substrate complex Daboia russelii russelii
0.0935
-
N-acetyltryptophan isoform L1, enzyme-substrate complex Daboia russelii russelii
0.1146
-
N-acetyltryptophan isoform L2, free enzyme Daboia russelii russelii
0.1275
-
N-acetyltryptophan isoform L1, free enzyme Daboia russelii russelii
0.2001
-
N-acetyltryptophan isoform L2, enzyme-substrate complex Daboia russelii russelii
0.3788
-
N-acetyl-L-tryptophan amide isoform L2, free enzyme Daboia russelii russelii
0.3846
-
N-acetyl-L-tryptophan amide isoform L1, enzyme-substrate complexe Daboia russelii russelii
0.5916
-
N-acetyl-L-tryptophan amide isoform L1, free enzyme Daboia russelii russelii
0.7448
-
N-acetyl-L-tryptophan amide isoform L2, enzyme-substrate complex Daboia russelii russelii