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Literature summary for 1.4.3.2 extracted from

  • De Vieira Santos, M.M.; SantAna, C.D.; Giglio, J.R.; da Silva, R.J.; Sampaio, S.V.; Soares, A.M.; Fecchio, D.
    Antitumoural effect of an L-amino acid oxidase isolated from Bothrops jararaca snake venom (2008), Basic Clin. Pharmacol. Toxicol., 102, 533-542.
    View publication on PubMed
Search for more literature for 1.4.3.2

Application

Application Comment Organism
medicine significantly inhibits Ehrlich ascites tumour growth and induces an influx of polymorphonuclear cells, as well as spontaneous liberation of H2O2 from peritoneal macrophages. Later, LAAO-I induces mononuclear influx and peritoneal macrophage spreading. Animals treated with LAAO-I show higher survival time Bothrops jararaca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Bothrops jararaca
125000
-
 gel filtration Bothrops jararaca

Organism

Organism UniProt Comment Textmining
Bothrops jararaca Q6TGQ9
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, affinity and hydrophobic interaction chromatography Bothrops jararaca

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Bothrops jararaca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + H2O + O2 high substrate activity Bothrops jararaca 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-leucine + H2O + O2 high substrate activity, most specific substrate Bothrops jararaca 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-methionine + H2O + O2 high substrate activity Bothrops jararaca 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-phenylalanine + H2O + O2 high substrate activity, most specific substrate Bothrops jararaca phenylpyruvate + NH3 + H2O2
-
 ?
additional information L-serine, L-proline, L-glycine, L-threonine and L-cysteine are not oxidized Bothrops jararaca ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Bothrops jararaca

Synonyms

Synonyms Comment Organism
LAAO-I
-
 Bothrops jararaca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 after heating to 45°C, LAAO activity gradually decreases. After heating at 60°C and 70°C for 10 min., 85% and 100% of activity is lost, respectively. Freezing at -70°C for 24 h causes a 50% loss of enzyme activity. After lyophilization, about 15% only of activity is retained Bothrops jararaca

pI Value

Organism Comment pI Value Maximum pI Value
Bothrops jararaca
-
-
 5