Literature summary for 1.4.3.2 extracted from

Yang, H.; Johnson, P.M.; Ko, K.C.; Kamio, M.; Germann, M.W.; Derby, C.D.; Tai, P.C.
Cloning, characterization and expression of escapin, a broadly antimicrobial FAD-containing L-amino acid oxidase from ink of the sea hare Aplysia californica (2005), J. Exp. Biol., 208, 3609-3622.

Search for more literature for 1.4.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
-	Aplysia californica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aplysia californica	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	gel filtration	Aplysia californica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Aplysia californica	native enzyme inhibits the growth of Gram-positive and Gram-negative bacteria, including marine bacteria, and also inhibits the growth of yeasts and fungi. In minimal media, enzyme is bacteriostatic and not bactericidal, but becomes bactericidal in media enriched with Tryptone Peptone	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Aplysia californica	Q6IWZ0	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	sequence contains one potential glycosylation site. Glycosylation is not essential for antimicrobial activity	Aplysia californica
proteolytic modification	sequence contains a signal peptide of 18 amino acids	Aplysia californica

Source Tissue

Source Tissue	Comment	Organism	Textmining
ink	-	Aplysia californica	-

Storage Stability

Storage Stability	Organism
no decrease in antimicrobial activity during storage for up to 5 months at room temperature	Aplysia californica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-arginine + H2O + O2	-	Aplysia californica	2-oxo-5-guanidinovaleric acid + NH3 + H2O2	-	?
additional information	native enzyme inhibits the growth of Gram-positive and Gram-negative bacteria, including marine bacteria, and also inhibits the growth of yeasts and fungi. In minimal media, enzyme is bacteriostatic and not bactericidal, but becomes bactericidal in media enriched with Tryptone Peptone	Aplysia californica	?	-	?
additional information	little or no activity with amino acids other than L-Arg, L-Lys	Aplysia californica	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 60000, SDS-PAGE	Aplysia californica

Synonyms

Synonyms	Comment	Organism
escapin	-	Aplysia californica

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Aplysia californica

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Release 2023.1 (January 2023)