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Literature summary for 1.4.3.2 extracted from

  • Kitani, Y.; Tsukamoto, C.; Zhang, G.; Nagai, H.; Ishida, M.; Ishizaki, S.; Shimakura, K.; Shiomi, K.; Nagashima, Y.
    Identification of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockfish Sebastes schlegeli (2007), FEBS J., 274, 125-136.
    View publication on PubMed
Search for more literature for 1.4.3.2

Application

Application Comment Organism
additional information LAO is involved in the innate immunity of fish skin. Shows potent antibacterial activity against fish pathogens, specifically Gram-negative bacteria such as Aeromonas hydrophila, Aeromonas salmonicida and Photobacterium damselae ssp. piscicida Sebastes schlegelii

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sebastes schlegelii
into the pT7Blue T-vector Sebastes schlegelii

Inhibitors

Inhibitors Comment Organism Structure
additional information catalase almost completely abolishs the antibacterial activity of SSAP, indicating that H2O2 is the mediator of the activity of SSAP Sebastes schlegelii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 L-lysine
-
 Sebastes schlegelii
0.19
-
 L-lysine 37°C Sebastes schlegelii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000, SDS-PAGE Sebastes schlegelii
53000
-
 2 * 53000, SDS-PAGE, 2 * 55261, sequence analysis Sebastes schlegelii
55261
-
 2 * 53000, SDS-PAGE, 2 * 55261, sequence analysis Sebastes schlegelii
120000
-
 gel filtration Sebastes schlegelii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sebastes schlegelii enzyme shows potent antibacterial activity against fish pathogens ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sebastes schlegelii
-
-
-
Sebastes schlegelii A1IGW6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein protein contains N-linked glycochains Sebastes schlegelii

Purification (Commentary)

Purification (Comment) Organism
-
 Sebastes schlegelii
to homogeneity by affinity chromatography, anion-exchange chromatography and gel filtration Sebastes schlegelii

Source Tissue

Source Tissue Comment Organism Textmining
skin skin mucus Sebastes schlegelii
-
skin mucus Sebastes schlegelii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.2
-
-
 Sebastes schlegelii
10.2
-
 37°C Sebastes schlegelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + H2O + O2
-
 Sebastes schlegelii 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
additional information enzyme shows potent antibacterial activity against fish pathogens Sebastes schlegelii ?
-
 ?
additional information enzyme uses only L-lysine as substrate and is ineffective with other proteinaceous amino acids or with D-lysine Sebastes schlegelii ?
-
 ?
additional information SSAP catalyzes oxidation of only L-lysine and is ineffective with any other proteinaceous amino acids. No LAO activity is detected when L-lysine is replaced with D-lysine Sebastes schlegelii ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Sebastes schlegelii
homodimer 2 * 53000, SDS-PAGE, 2 * 55261, sequence analysis Sebastes schlegelii

Synonyms

Synonyms Comment Organism
LAO
-
 Sebastes schlegelii
Sebastes schlegeli antibacterial protein
-
 Sebastes schlegelii
SSAP
-
 Sebastes schlegelii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.4
-
 L-lysine
-
 Sebastes schlegelii
20.4
-
 L-lysine 37°C Sebastes schlegelii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Sebastes schlegelii