General Stability | Organism |
---|---|
freezing at -18°C without addition of glycerol inactivates the enzyme | Rhodococcus opacus |
stability of the enzyme can be increased by storage or incubation of the enzyme in glycine/NaOH buffer | Rhodococcus opacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycine | activity strongly decreased in glycine/NaOH-buffer, competitive inhibition, no inhibition, when 10 mM L-phenylanalnine is used as substrate | Rhodococcus opacus | |
L-arginine | pH 7.6, 30°C | Rhodococcus opacus | |
L-citrulline | pH 7.6, 30°C | Rhodococcus opacus | |
L-leucine | pH 7.6, 30°C | Rhodococcus opacus | |
L-lysine | pH 7.6, 30°C | Rhodococcus opacus | |
L-methionine | pH 7.6, 30°C | Rhodococcus opacus | |
L-norleucine | pH 7.6, 30°C | Rhodococcus opacus | |
L-ornithine | pH 7.6, 30°C | Rhodococcus opacus | |
L-phenylalanine | pH 7.6, 30°C | Rhodococcus opacus | |
L-serine | pH 7.6, 30°C | Rhodococcus opacus | |
L-tyrosine | pH 7.6, 30°C | Rhodococcus opacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
L-lysine | pH 7.6, 30°C | Rhodococcus opacus | |
0.019 | - |
L-tyrosine | pH 7.6, 30°C | Rhodococcus opacus | |
0.022 | - |
L-phenylalanine | pH 7.6, 30°C | Rhodococcus opacus | |
0.026 | - |
L-norleucine | pH 7.6, 30°C | Rhodococcus opacus | |
0.026 | - |
L-citrulline | pH 7.6, 30°C | Rhodococcus opacus | |
0.028 | - |
L-leucine | pH 7.6, 30°C | Rhodococcus opacus | |
0.028 | - |
L-asparagine | pH 7.6, 30°C | Rhodococcus opacus | |
0.034 | - |
L-ornithine | pH 7.6, 30°C | Rhodococcus opacus | |
0.039 | - |
L-methionine | pH 7.6, 30°C | Rhodococcus opacus | |
0.07 | - |
L-arginine | pH 7.6, 30°C | Rhodococcus opacus | |
0.085 | - |
L-glutamine | pH 7.6, 30°C | Rhodococcus opacus | |
0.274 | - |
L-alanine | pH 7.6, 30°C | Rhodococcus opacus | |
0.411 | - |
L-Glutamic acid | pH 7.6, 30°C | Rhodococcus opacus | |
1.36 | - |
L-serine | pH 7.6, 30°C | Rhodococcus opacus | |
3.73 | - |
L-valine | pH 7.6, 30°C | Rhodococcus opacus | |
5.11 | - |
L-isoleucine | pH 7.6, 30°C | Rhodococcus opacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules | Rhodococcus opacus |
54000 | - |
2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules | Rhodococcus opacus |
99000 | - |
gel filtration | Rhodococcus opacus |
104000 | - |
MALDI-TOF/MS | Rhodococcus opacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | Q8VPD4 | DSM 43250, one of the best L-AAO producer | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, recovery of 68% and 144fold | Rhodococcus opacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
homogenous enzyme, L-alanine as susbtrate, 30°C, pH 7.6 | Rhodococcus opacus |
Storage Stability | Organism |
---|---|
-18°C, 50 mM glycine/NaOH buffer, pH 8.6, 50% glycerol, stable for several months, withou glycerol inactivation | Rhodococcus opacus |
4°C, 50 mM glycine/NaOH buffer, pH 8.6, stable for several months | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-3-(1-pyridyl)alanine + H2O + O2 | 24% of the activity compared to L-alanine | Rhodococcus opacus | 3-(1-pyridyl)pyruvate + NH3 + H2O2 | - |
? | |
DL-4-fluorophenylalanine + H2O + O2 | 25% of the activity compared to L-alanine | Rhodococcus opacus | (4-fluorophenyl)pyruvate + NH3 + H2O2 | - |
? | |
DL-homophenylalanine + H2O + O2 | 50% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxo-4-phenylbutanoic acid + NH3 + H2O2 | - |
? | |
DL-norleucine + H2O + O2 | 73% of the activity compared to L-alanine | Rhodococcus opacus | alpha-ketocaproate + NH3 + H2O2 | - |
? | |
DL-O-methylserine + H2O + O2 | 99% of the activity compared to L-alanine | Rhodococcus opacus | ? + NH3 + H2O2 | - |
? | |
DL-phenylseine + H2O + O2 | 8% of the activity compared to L-alanine | Rhodococcus opacus | ? + NH3 + H2O2 | - |
? | |
L-2,3-diaminopropionic acid + H2O + O2 | 71% of the activity compared to L-alanine | Rhodococcus opacus | ? + NH3 + H2O2 | - |
? | |
L-2,4-diaminobutyric acid + H2O + O2 | 100% of the activity compared to L-alanine | Rhodococcus opacus | 4-amino-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-2-amino-4,4-dimethylvaleric acid + H2O + O2 | 46% of the activity compared to L-alanine | Rhodococcus opacus | 4,4-dimethyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-2-amino-5,5-dimethylhexanoic acid + H2O + O2 | 44% of the activity compared to L-alanine | Rhodococcus opacus | 5,5-dimethyl-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
L-2-chlorophenylalanine + H2O + O2 | 24% of the activity compared to L-alanine | Rhodococcus opacus | (2-chlorophenyl)pyruvate + NH3 + H2O2 | - |
? | |
L-3-(1-naphthyl)alanine + H2O + O2 | 109% of the activity compared to L-alanine | Rhodococcus opacus | 3-(1-naphthyl)pyruvate + NH3 + H2O2 | - |
? | |
L-4-aminophenylalanine + H2O + O2 | 16% of the activity compared to L-alanine | Rhodococcus opacus | (4-aminophenyl)pyruvate + NH3 + H2O2 | - |
? | |
L-4-chlorophenylalanine + H2O + O2 | 9% of the activity compared to L-alanine | Rhodococcus opacus | (4-chlorophenyl)pyruvate + NH3 + H2O2 | - |
? | |
L-4-nitrophenylalanine + H2O + O2 | 30% of the activity compared to L-alanine | Rhodococcus opacus | (4-nitrophenyl)pyruvate + NH3 + H2O2 | - |
? | |
L-alanine + H2O + O2 | highly enantioselective enzyme with a broad substrate specificity | Rhodococcus opacus | 2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
L-arginine + H2O + O2 | 70% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-asparagine + H2O + O2 | 97% of the activity compared to L-alanine | Rhodococcus opacus | alpha-ketosuccinamic acid + NH3 + H2O2 | - |
? | |
L-aspartic acid + H2O + O2 | 11% of the activity compared to L-alanine | Rhodococcus opacus | oxaloacetate + NH3 + H2O2 | - |
? | |
L-citrulline + H2O + O2 | 74% of the activity compared to L-alanine | Rhodococcus opacus | N-carbamoyl-5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-cysteic acid + H2O + O2 | 34% of the activity compared to L-alanine | Rhodococcus opacus | ? + NH3 + H2O2 | - |
? | |
L-cystine + H2O + O2 | 104% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxo-3-mercaptopropanoic acid + NH3 + H2O2 | - |
? | |
L-glutamic acid + H2O + O2 | 52% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamine + H2O + O2 | 52% of the activity compared to L-alanine | Rhodococcus opacus | 5-amino-2,5-dioxopentanoate + NH3 + H2O2 | - |
? | |
L-histidine + H2O + O2 | 36% of the activity compared to L-alanine | Rhodococcus opacus | 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2 | - |
? | |
L-homocysteine thiolactone + H2O + O2 | 23% of the activity compared to L-alanine | Rhodococcus opacus | ? + NH3 + H2O2 | - |
? | |
L-homoserine lactone + H2O + O2 | 66% of the activity compared to L-alanine | Rhodococcus opacus | ? + NH3 + H2O2 | - |
? | |
L-isoleucine + H2O + O2 | 45% of the activity compared to L-alanine | Rhodococcus opacus | alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | 68% of the activity compared to L-alanine | Rhodococcus opacus | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-lysine + H2O + O2 | 55% of the activity compared to L-alanine | Rhodococcus opacus | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | 72% of the activity compared to L-alanine | Rhodococcus opacus | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-norleucine + H2O + O2 | - |
Rhodococcus opacus | 2-oxohexanoate + NH3 + H2O2 | - |
r | |
L-ornithine + H2O + O2 | 117% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxo-5-aminopentanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | 53% of the activity compared to L-alanine | Rhodococcus opacus | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-phenylglycine + H2O + O2 | 5% of the activity compared to L-alanine | Rhodococcus opacus | phenylacetate + NH3 + H2O2 | - |
? | |
L-serine + H2O + O2 | 72% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | 12% of the activity compared to L-alanine | Rhodococcus opacus | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | 54% of the activity compared to L-alanine | Rhodococcus opacus | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
L-valine + H2O + O2 | 32% of the activity compared to L-alanine | Rhodococcus opacus | 2-oxoisovalerate + NH3 + H2O2 | - |
? | |
Nepsilon-acetyl-L-lysine + H2O + O2 | 46% of the activity compared to L-alanine | Rhodococcus opacus | 6-acetylamino-2-oxohexanoic acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules | Rhodococcus opacus |
Synonyms | Comment | Organism |
---|---|---|
L-AAO | - |
Rhodococcus opacus |
L-amino acid:O2 oxidoreductase (deaminating) | - |
Rhodococcus opacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | inactivation in 50 mM triethylamine/HCl buffer, pH 7.6 in the same range as 50 mM potassium-phosphate buffer pH 6.6 | Rhodococcus opacus |
30 | - |
incubation for 30 min with 0.28 M H2O2 results in 50% inactivation | Rhodococcus opacus |
37 | - |
t1/2: 35 min, increases more than 20fold by incubating the L-AAO in glycine/NaOH buffer: 938 min, potassium phosphate buffer t1/2: 44 min | Rhodococcus opacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | for 10 mM L-alanine, L-leucine, L-phenylalanine, the pH-profiles are almost identical | Rhodococcus opacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | L-phenylalanine as substrate, pH 4: ca. 10% of the activity, pH 11: ca. 60% of the activity | Rhodococcus opacus |
4.5 | 10.5 | L-alanine as substrate, pH 4.5, ca. 3% of the activity, pH 10.5, ca. 30% of the activity | Rhodococcus opacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | a homodimer complex containing 2 FAD molecules | Rhodococcus opacus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.06 | - |
L-tyrosine | pH 7.6, 30°C | Rhodococcus opacus | |
2.28 | - |
L-phenylalanine | pH 7.6, 30°C | Rhodococcus opacus | |
2.91 | - |
L-norleucine | pH 7.6, 30°C | Rhodococcus opacus | |
4.5 | - |
glycine | pH 7.6, 30°C | Rhodococcus opacus | |
5.42 | - |
L-arginine | pH 7.6, 30°C | Rhodococcus opacus | |
5.69 | - |
L-leucine | pH 7.6, 30°C | Rhodococcus opacus | |
6.84 | - |
L-methionine | pH 7.6, 30°C | Rhodococcus opacus | |
12.5 | - |
L-ornithine | pH 7.6, 30°C | Rhodococcus opacus | |
20.6 | - |
L-citrulline | pH 7.6, 30°C | Rhodococcus opacus | |
63.7 | - |
L-lysine | pH 7.6, 30°C | Rhodococcus opacus | |
192 | - |
L-serine | pH 7.6, 30°C | Rhodococcus opacus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus opacus | isoelectric focusing, using a narrow pH-range of 4-6 | - |
4.8 |