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Literature summary for 1.4.3.19 extracted from

  • Jamil, F.; Afza Gardner, Q.T.; Bashir, Q.; Rashid, N.; Akhtar, M.
    Mechanistic and stereochemical studies of glycine oxidase from Bacillus subtilis strain R5 (2010), Biochemistry, 49, 7377-7383.
    View publication on PubMed
Search for more literature for 1.4.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40762
-
 x * 40763, recombinant enzyme, mass spectrometry, x * 40762, sequence calculation Bacillus subtilis
40763
-
 x * 40763, recombinant enzyme, mass spectrometry, x * 40762, sequence calculation Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycine + O2 + H2O Bacillus subtilis
-
 glyoxylate + H2O2 + NH3
-
 ?
glycine + O2 + H2O Bacillus subtilis R5
-
 glyoxylate + H2O2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis R5
-
-
-

Reaction

Reaction Comment Organism Reaction ID
glycine + H2O + O2 = glyoxylate + NH3 + H2O2 reaction mechanism involving an FADH2-imino acid complex, overview Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + O2 + H2O
-
 Bacillus subtilis glyoxylate + H2O2 + NH3
-
 ?
glycine + O2 + H2O using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview Bacillus subtilis glyoxylate + H2O2 + NH3
-
 ?
glycine + O2 + H2O
-
 Bacillus subtilis R5 glyoxylate + H2O2 + NH3
-
 ?
glycine + O2 + H2O using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview Bacillus subtilis R5 glyoxylate + H2O2 + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 40763, recombinant enzyme, mass spectrometry, x * 40762, sequence calculation Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD the reaction mechanism involves an FADH2-imino acid complex Bacillus subtilis