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Literature summary for 1.4.3.19 extracted from

  • Martinez-Martinez, I.; Navarro-Fernandez, J.; Garcia-Carmona, F.; Takami, H.; Sanchez-Ferrer, A.
    Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426 (2008), Proteins Struct. Funct. Bioinform., 70, 1429-1441.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus kaustophilus HTA426

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
N-ethylglycine pH 8.5, 37°C Geobacillus kaustophilus HTA426
0.25
-
glycine pH 8.5, 37°C Geobacillus kaustophilus HTA426
0.45
-
sarcosine pH 8.5, 37°C Geobacillus kaustophilus HTA426
0.52
-
glycine-ethyl-ester pH 8.5, 37°C Geobacillus kaustophilus HTA426
9.3
-
D-proline pH 8.5, 37°C Geobacillus kaustophilus HTA426
20.6
-
D-alanine pH 8.5, 37°C Geobacillus kaustophilus HTA426
221.8
-
D-pipecolate pH 8.5, 37°C Geobacillus kaustophilus HTA426

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
4 * 43000, SDS-PAGE Geobacillus kaustophilus HTA426
160000
-
gel filtration Geobacillus kaustophilus HTA426

Organism

Organism UniProt Comment Textmining
Geobacillus kaustophilus HTA426
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.85
-
pH 8.5, 37°C Geobacillus kaustophilus HTA426

Storage Stability

Storage Stability Organism
-80°C, 75 mM sodium diphosphate, pH 8.5, 10% glycerol, stable for months Geobacillus kaustophilus HTA426

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
Geobacillus kaustophilus HTA426 ?
-
?
D-Ala + H2O + O2
-
Geobacillus kaustophilus HTA426 ? + NH3 + H2O2
-
?
D-arginine + H2O + O2
-
Geobacillus kaustophilus HTA426 ?
-
?
D-aspartate + H2O + O2
-
Geobacillus kaustophilus HTA426 ?
-
?
D-methionine + H2O + O2
-
Geobacillus kaustophilus HTA426 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
-
?
D-norvaline + H2O + O2
-
Geobacillus kaustophilus HTA426 2-oxopentanoic acid + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2
-
Geobacillus kaustophilus HTA426 phenylpyruvate + NH3 + H2O2
-
?
D-pipecolate + H2O + O2
-
Geobacillus kaustophilus HTA426 ?
-
?
D-Pro + H2O + O2
-
Geobacillus kaustophilus HTA426 ? + NH3 + H2O2
-
?
D-valine + H2O + O2
-
Geobacillus kaustophilus HTA426 3-methyl-2-oxobutanoic acid + NH3 + H2O2
-
?
glycine + H2O + O2
-
Geobacillus kaustophilus HTA426 glyoxylate + NH3 + H2O2
-
?
glycine-ethyl-ester + H2O + O2
-
Geobacillus kaustophilus HTA426 ? + H2O2
-
?
additional information substrate preference for amines of small size, like glycine, sarcosine, N-ethyl-glycine, and glycine-ethylester Geobacillus kaustophilus HTA426 ?
-
?
N-ethylglycine + H2O + O2
-
Geobacillus kaustophilus HTA426 glyoxylate + ethylamine + H2O2
-
?
sarcosine + H2O + O2
-
Geobacillus kaustophilus HTA426 glyoxylate + methylamine + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, SDS-PAGE Geobacillus kaustophilus HTA426

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
74
-
-
Geobacillus kaustophilus HTA426

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
activation during the first 15 min, with slow decrease thereafter Geobacillus kaustophilus HTA426

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life 0.6 h Geobacillus kaustophilus HTA426

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Geobacillus kaustophilus HTA426