KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
O2 | pH 9.0, 25°C | Bacillus subtilis | |
0.15 | - |
O2 | pH 6.5, 25°C | Bacillus subtilis | |
0.25 | - |
O2 | pH 7.5, 25°C | Bacillus subtilis | |
0.28 | - |
O2 | pH 8.5, 25°C | Bacillus subtilis | |
0.4 | - |
O2 | pH 9.5, 25°C | Bacillus subtilis | |
0.45 | - |
O2 | pH 7.0, 25°C | Bacillus subtilis | |
3.8 | - |
glycine | pH 8.5, 25°C | Bacillus subtilis | |
5.4 | - |
glycine | pH 7.5, 25°C | Bacillus subtilis | |
5.4 | - |
glycine | pH 9.0, 25°C | Bacillus subtilis | |
5.6 | - |
glycine | pH 7.0, 25°C | Bacillus subtilis | |
9.2 | - |
glycine | pH 6.5, 25°C | Bacillus subtilis | |
14.7 | - |
glycine | pH 9.5, 25°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31616 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + NH3 + H2O2 | free glycine oxidase forms the anionic red semiquinone upon photoreduction.This species is thermodynamically stable, as indicated by the large separation of the two single-electron reduction potentials of DeltaE 290 mV. The first potential is pH-independent, while the second is dependent. The midpoint reduction potential exhibits a 23.4 mV/pH unit slope, which is consistent with an overall two-electrons/one-proton transfer in the reduction to yield anionic reduced flavin. In the presence of glycolate and at pH 7.5 the potential for the semiquinone-reduced enzyme couple is shifted positively by about 160 mV, this favors a two-electron transfer compared to the free enzyme. Binding of glycolate and sulfite is also affected by pH | ? | |
sarcosine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + methylamine + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
glycine | pH 6.5, 25°C | Bacillus subtilis | |
2.4 | - |
glycine | pH 7.0, 25°C | Bacillus subtilis | |
3 | - |
glycine | pH 9.5, 25°C | Bacillus subtilis | |
3.6 | - |
glycine | pH 8.0, 25°C | Bacillus subtilis | |
4 | - |
glycine | pH 8.5, 25°C | Bacillus subtilis | |
4.1 | - |
glycine | pH 9.0, 25°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | the tetrameric oligomeric state of the holoenzyme is not affected within this range | Bacillus subtilis |
7 | 8 | optimal range for stability | Bacillus subtilis |