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Literature summary for 1.4.3.14 extracted from

  • Amano, M.; Mizuguchi, H.; Sano, T.; Kondo, H.; Shinyashiki, K.; Inagaki, J.; Tamura, T.; Kawaguchi, T.; Kusakabe, H.; Imada, K.; Inagaki, K.
    Recombinant expression, molecular characterization and crystal structure of antitumor enzyme, L-lysine alpha-oxidase from Trichoderma viride (2015), J. Biochem., 157, 549-559 .
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme severely inhibits growth of cancer cells but shows relatively low cytotoxicity fo normal cells Trichoderma viride

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans strain TK24 Trichoderma viride

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1M HEPES-NaOH (pH 7.6), 2% (w/v) PEG-400 and 1.8 M ammonium sulfate Trichoderma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Trichoderma viride
-
6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O Trichoderma viride Y244-2
-
6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
-
-
Trichoderma viride Y244-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl 650M column chromatography Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + O2 + H2O 6.3% activity compared to L-lysine Trichoderma viride 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
?
L-arginine + O2 + H2O 6.3% activity compared to L-lysine Trichoderma viride Y244-2 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O
-
Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O 100% activity Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O
-
Trichoderma viride Y244-2 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O 100% activity Trichoderma viride Y244-2 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-ornithine + O2 + H2O 18.3% activity compared to L-lysine Trichoderma viride 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
?
L-ornithine + O2 + H2O 18.3% activity compared to L-lysine Trichoderma viride Y244-2 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
?
L-phenylalanine + O2 + H2O 1.7% activity compared to L-lysine Trichoderma viride phenylpyruvate + NH3 + H2O2
-
?
L-phenylalanine + O2 + H2O 1.7% activity compared to L-lysine Trichoderma viride Y244-2 phenylpyruvate + NH3 + H2O2
-
?
L-tyrosine + O2 + H2O 1.4% activity compared to L-lysine Trichoderma viride 4-hydroxyphenylpyruvate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 56000, SDS-PAGE Trichoderma viride

Synonyms

Synonyms Comment Organism
L-lysine alpha-oxidase
-
Trichoderma viride
LysOx
-
Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trichoderma viride

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trichoderma viride