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Literature summary for 1.4.3.11 extracted from

  • Kusakabe, H.; Midorikawa, Y.; Fujishima, T.; Kuninaka, A.; Yoshino, H.
    Purification and properties of a new enzyme, L-glutamate oxidase, from Streptomyces sp. X-119-6 grown on wheat bran (1983), Agric. Biol. Chem., 47, 1323-1328.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ no inhibition by CuCl2 Streptomyces sp.
PCMB
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9000
-
alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE Streptomyces sp.
19000
-
alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE Streptomyces sp.
44000
-
alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE Streptomyces sp.
140000
-
gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information wheat bran culture Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55.1
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp + O2 + H2O at 0.6% of the activity with L-glutamate, at pH 7.4, no measurable activity at pH 6.0 Streptomyces sp. 2-oxosuccinamic acid + NH3 + H2O2
-
?
L-glutamate + O2 + H2O
-
Streptomyces sp. 2-oxoglutarate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
hexamer alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 5.0-10.5, 1 h, stable Streptomyces sp.
60
-
pH 5.5-7.0, 15 min, stable Streptomyces sp.
85
-
pH 5.5, 15 min, 50% loss of activity Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 10 pH 4.0: about 50% of maximal activity pH 10.0: about 40% of maximal activity Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
FAD contains 2 mol of FAD per mol of enzyme Streptomyces sp.