Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | no inhibition by CuCl2 | Streptomyces sp. | |
PCMB | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9000 | - |
alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE | Streptomyces sp. |
19000 | - |
alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE | Streptomyces sp. |
44000 | - |
alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE | Streptomyces sp. |
140000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | wheat bran culture | Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55.1 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asp + O2 + H2O | at 0.6% of the activity with L-glutamate, at pH 7.4, no measurable activity at pH 6.0 | Streptomyces sp. | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
L-glutamate + O2 + H2O | - |
Streptomyces sp. | 2-oxoglutarate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2beta2gamma2, 2 * 44000 + 2 * 19000 + 2 * 9000, SDS-PAGE | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 5.0-10.5, 1 h, stable | Streptomyces sp. |
60 | - |
pH 5.5-7.0, 15 min, stable | Streptomyces sp. |
85 | - |
pH 5.5, 15 min, 50% loss of activity | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | pH 4.0: about 50% of maximal activity pH 10.0: about 40% of maximal activity | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 2 mol of FAD per mol of enzyme | Streptomyces sp. |