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Literature summary for 1.4.3.11 extracted from

  • Boehmer, A.; Mueller, A.; Passarge, M.; Liebs, P.; Honeck, H.; Mueller, H.G.
    A novel L-glutamate oxidase from Streptomyces endus. Purification and properties (1989), Eur. J. Biochem., 182, 327-332.
    View publication on PubMed
Search for more literature for 1.4.3.11

Inhibitors

Inhibitors Comment Organism Structure
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazol 1 mM, 18% loss of activity Streptomyces hygroscopicus subsp. hygroscopicus
Ag+ 0.1 mM AgNO3, 94% loss of activity Streptomyces hygroscopicus subsp. hygroscopicus
Hg2+ 0.1 mM HgCl2, complete inactivation Streptomyces hygroscopicus subsp. hygroscopicus
PCMB 1 mM, 16% loss of activity Streptomyces hygroscopicus subsp. hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 L-glutamate pH 7.0 Streptomyces hygroscopicus subsp. hygroscopicus
1.86
-
 O2
-
 Streptomyces hygroscopicus subsp. hygroscopicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces hygroscopicus subsp. hygroscopicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Streptomyces hygroscopicus subsp. hygroscopicus
90000
-
 gel filtration Streptomyces hygroscopicus subsp. hygroscopicus

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus subsp. hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces hygroscopicus subsp. hygroscopicus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Streptomyces hygroscopicus subsp. hygroscopicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
-
 Streptomyces hygroscopicus subsp. hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-benzyl-L-glutamate + O2 + H2O 13% of the activity with L-glutamate Streptomyces hygroscopicus subsp. hygroscopicus 4-benzyl-2-oxoglutarate + NH3 + H2O2
-
 ?
L-glutamate + O2 + H2O high specificity for L-glutamate Streptomyces hygroscopicus subsp. hygroscopicus 2-oxoglutarate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Streptomyces hygroscopicus subsp. hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 45
-
 Streptomyces hygroscopicus subsp. hygroscopicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 25°C: about 75% of maximal activity, 60°C: about 55% of maximal activity Streptomyces hygroscopicus subsp. hygroscopicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 7.0, 1 h, stable Streptomyces hygroscopicus subsp. hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
 Streptomyces hygroscopicus subsp. hygroscopicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 30% of maximal activity, pH 8.5: about 80% of maximal activity Streptomyces hygroscopicus subsp. hygroscopicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 37°C, 15 min, stable Streptomyces hygroscopicus subsp. hygroscopicus

Cofactor

Cofactor Comment Organism Structure
FAD one molecule of noncovalently bound FAD per subunit Streptomyces hygroscopicus subsp. hygroscopicus