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Literature summary for 1.4.3.1 extracted from

  • Katane, M.; Hanai, T.; Furuchi, T.; Sekine, M.; Homma, H.
    Hyperactive mutants of mouse D-aspartate oxidase: mutagenesis of the active site residue serine 308 (2008), Amino Acids, 35, 75-82.
    View publication on PubMed
Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3)pLysS Mus musculus

Protein Variants

Protein Variants Comment Organism
S308A has a significantly higher catalytic efficiency towards D-Asp and N-methyl-D-Asp, and a higher affinity for FAD compared to the wild type enzyme Mus musculus
S308G has a significantly higher catalytic efficiency towards D-Asp and N-methyl-D-Asp, and a higher affinity for FAD compared to the wild type enzyme Mus musculus
S308Y has a significantly lower catalytic efficiency towards D-Asp and N-methyl-D-Asp, and a lower affinity for FAD compared to the wild type enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 D-Aspartate mutant enzyme S308G fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
1.78
-
 D-Aspartate mutant enzyme S308A fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
1.95
-
 N-Methyl-D-aspartate mutant enzyme S308G fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
2.37
-
 D-Aspartate His-tagged mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
2.67
-
 N-Methyl-D-aspartate His-tagged mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
4.23
-
 N-Methyl-D-aspartate mutant enzyme S308A fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
4.87
-
 D-Aspartate wild type enzyme fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
6.59
-
 N-Methyl-D-aspartate wild type enzyme fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
7.3
-
 D-Aspartate His-tagged wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
7.63
-
 D-Aspartate mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
7.81
-
 N-Methyl-D-aspartate mutant enzyme S308Y fused N-terminally with glutathione S-transferase, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus
8.02
-
 N-Methyl-D-aspartate His-tagged wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q0E9Q7
-
-

Purification (Commentary)

Purification (Comment) Organism
His GraviTrap column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate Mus musculus
0.11
-
 mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate Mus musculus
0.24
-
 mutant enzyme S308A, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate Mus musculus
0.55
-
 mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate Mus musculus
2.37
-
 mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate Mus musculus
2.54
-
 mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate Mus musculus
4.31
-
 wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate Mus musculus
4.32
-
 wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate Mus musculus
5.55
-
 mutant enzyme S308A, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate Mus musculus
5.92
-
 mutant enzyme S308A, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate Mus musculus
9.24
-
 mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate Mus musculus
13.1
-
 mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aspartate + H2O + O2
-
 Mus musculus oxaloacetate + NH3 + H2O2
-
 ?
N-methyl-D-aspartate + H2O + O2
-
 Mus musculus oxaloacetate + CH3NH2 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
DASPO
-
 Mus musculus