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Literature summary for 1.4.3.1 extracted from

  • Katane, M.; Seida, Y.; Sekine, M.; Furuchi, T.; Homma, H.
    Caenorhabditis elegans has two genes encoding functional D-aspartate oxidases (2007), FEBS J., 274, 137-149.
    View publication on PubMed
Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
the C47A10.5 gene product is expressed in Escherichia coli as recombinant protein with an N-terminal His-tag Caenorhabditis elegans
the F18E3.7a gene product is expressed in Escherichia coli as recombinant protein with an N-terminal His-tag Caenorhabditis elegans
the His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 D-glutamate 37°C, pH 8.3, C47A10.5 gene product Caenorhabditis elegans
0.23
-
 D-glutamate recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
0.25
-
 D-glutamate 37°C, pH 8.3, F18E3.7a gene product Caenorhabditis elegans
0.25
-
 D-glutamate recombinant F18Ep protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
0.38
-
 D-Aspartate 37°C, pH 8.3, F18E3.7a gene product Caenorhabditis elegans
0.38
-
 D-asparagine recombinant F18Ep protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
0.84
-
 N-Methyl-D-aspartate 37°C, pH 8.3, C47A10.5 gene product Caenorhabditis elegans
0.84
-
 N-methyl-D-asparagine recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
1.84
-
 N-Methyl-D-aspartate 37°C, pH 8.3, F18E3.7a gene product Caenorhabditis elegans
1.84
-
 N-methyl-D-asparagine recombinant F18Ep protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
2.02
-
 D-Aspartate 37°C, pH 8.3, C47A10.5 gene product Caenorhabditis elegans
2.02
-
 D-asparagine recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37610
-
 F18Ep, calculated from sequence of cDNA Caenorhabditis elegans
37640
-
 C47Ap, calculated from sequence of cDNA Caenorhabditis elegans
41770
-
 His-tagged F18Ep, calculated from sequence of cDNA Caenorhabditis elegans
41790
-
 His-tagged C47Ap, calculated from sequence of cDNA Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Caenorhabditis elegans
-
 at least two genes encoding functional DASPOs (C47Ap and F18Ep). The two DASPOs differ in their substrate specificities and possibly also in their subcellular localization
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Chelating HP column chromatography Caenorhabditis elegans
recombinant C47A10.5 gene product Caenorhabditis elegans
recombinant F18E3.7a gene product Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2 C47Ap exhibits 4.5% activity and F18Ep exhibits 2.8% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?
D-arginine + H2O + O2 C47Ap exhibit less than 0.1% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?
D-asparagine + H2O + O2 C47Ap exhibits 17% activity and F18Ep exhibits 6.1% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?
D-aspartate + H2O + O2 100% activity Caenorhabditis elegans oxaloacetate + NH3 + H2O2
-
 ?
D-aspartate + H2O + O2 at least two genes encoding functional DASPOs (C47Ap and F18Ep). The two DASPOs differ in their substrate specificities and possibly also in their subcellular localization Caenorhabditis elegans oxaloacetate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2 activity is 2.5fold higher than with D-Asp, F18E3.7a gene product Caenorhabditis elegans 2-oxoglutarate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2 activity is 67% of the activity with D-Asp, C47A10.5 gene product Caenorhabditis elegans 2-oxoglutarate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2 at least two genes encoding functional DASPOs (C47Ap and F18Ep). The two DASPOs differ in their substrate specificities and possibly also in their subcellular localization Caenorhabditis elegans 2-oxoglutarate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2 C47Ap exhibits 247% activity and F18Ep exhibits 67% activity compared to D-aspartate as substrate Caenorhabditis elegans 2-oxoglutarate + NH3 + H2O2
-
 ?
D-methionine + H2O + O2 C47Ap exhibits 3.8% activity and F18Ep exhibits 2.4% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?
L-asparagine + H2O + O2 C47Ap exhibits 2.6% activity and F18Ep exhibits less than 0.1% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?
L-glutamine + H2O + O2 C47Ap exhibits 1.3% activity and F18Ep exhibits 0.3% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?
additional information no detectable activity with L-alanine, L-methionine, and L-arginine Caenorhabditis elegans ?
-
 ?
N-methyl-D-asparagine + H2O + O2
-
 Caenorhabditis elegans ?
-
 r
N-methyl-D-aspartate + H2O + O2 activity is 1.1fold higher than with D-Asp, C47A10.5 gene product Caenorhabditis elegans oxaloacetate + CH3NH2 + H2O2
-
 ?
N-methyl-D-aspartate + H2O + O2 activity is 3.1fold higher than with D-Asp, F18E3.7a gene product Caenorhabditis elegans oxaloacetate + CH3NH2 + H2O2
-
 ?
N-methyl-D-aspartate + H2O + O2 C47Ap exhibits 313% activity and F18Ep exhibits 110% activity compared to D-aspartate as substrate Caenorhabditis elegans oxaloacetate + CH3NH2 + H2O2
-
 ?
N-methyl-L-asparagine + H2O + O2 C47Ap exhibits 6.7% activity and F18Ep exhibits 1.1% activity compared to D-aspartate as substrate Caenorhabditis elegans ?
-
 ?

Synonyms

Synonyms Comment Organism
C47A10.5 gene product
-
 Caenorhabditis elegans
C47Ap
-
 Caenorhabditis elegans
C47Ap the protein with DASPO activity shows distinct kinetic properties against D-Asp, D-Glu, and N-methyl-D-Asp Caenorhabditis elegans
DASPO
-
 Caenorhabditis elegans
F18E3.7a gene product
-
 Caenorhabditis elegans
F18Ep
-
 Caenorhabditis elegans
F18Ep the protein with DASPO activity shows distinct kinetic properties against D-Asp, D-Glu, and N-methyl-D-Asp Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.11
-
 D-glutamate 37°C, pH 8.3, F18E3.7a gene product Caenorhabditis elegans
2.11
-
 D-glutamate recombinant F18Ep protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
3
-
 N-Methyl-D-aspartate 37°C, pH 8.3, F18E3.7a gene product Caenorhabditis elegans
3
-
 N-methyl-D-asparagine recombinant F18Ep protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
3.07
-
 D-Aspartate 37°C, pH 8.3, F18E3.7a gene product Caenorhabditis elegans
3.07
-
 D-asparagine recombinant F18Ep protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
3.13
-
 D-Aspartate 37°C, pH 8.3, C47A10.5 gene product Caenorhabditis elegans
4.29
-
 D-asparagine recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
5.31
-
 D-glutamate 37°C, pH 8.3, C47A10.5 gene product Caenorhabditis elegans
5.31
-
 D-glutamate recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans
6.13
-
 N-Methyl-D-aspartate 37°C, pH 8.3, C47A10.5 gene product Caenorhabditis elegans
6.13
-
 N-methyl-D-asparagine recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Caenorhabditis elegans