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Literature summary for 1.4.3.1 extracted from

  • Dixon, M.; Kenworthy, P.
    D-aspartate oxidase of kidney (1967), Biochim. Biophys. Acta, 146, 54-76.
    View publication on PubMed
Search for more literature for 1.4.3.1

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutaric acid at 6.67 mM 64% inhibition Oryctolagus cuniculus
adipic acid at 6.67 mM 15% inhibition Oryctolagus cuniculus
Citric acid at 6.67 mM 29% inhibition Oryctolagus cuniculus
D-malate 90% inhibition at 0.33 mM Oryctolagus cuniculus
D-Tartaric acid at 6.67 mM 77% inhibition Oryctolagus cuniculus
Fumaric acid at 6.67 mM 78% inhibition Oryctolagus cuniculus
Glutaric acid at 6.67 mM 62% inhibition Oryctolagus cuniculus
L-leucine 6% inhibition at 6.67 mM Oryctolagus cuniculus
L-Malic acid at 6.67 mM 79% inhibition Oryctolagus cuniculus
L-Tartaric acid at 6.67 mM 24% inhibition Oryctolagus cuniculus
L-valine 6% inhibition at 6.67 mM Oryctolagus cuniculus
malonate at 0.66 mM 78% inhibition Oryctolagus cuniculus
oxalic acid at 6.67 mM 40% inhibition Oryctolagus cuniculus
oxaloacetic acid at 6.67 mM 90% inhibition Oryctolagus cuniculus
succinic acid at 6.67 mM 66% inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
 D-Aspartate O2 as acceptor Oryctolagus cuniculus
5.2
-
 D-Aspartate ferricyanide as acceptor Oryctolagus cuniculus
5.7
-
 D-glutamate ferricyanide as acceptor Oryctolagus cuniculus
166
-
 D-glutamate O2 as acceptor Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-aspartate + H2O + O2 Oryctolagus cuniculus
-
 oxaloacetate + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
 ferricyanide as electron acceptor Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aspartate + H2O + O2
-
 Oryctolagus cuniculus oxaloacetate + NH3 + H2O2
-
 ?
D-aspartate + H2O + O2 ferricyanide and 2,6-dichlorophenolindophenol can also act as electron acceptors instead of O2 Oryctolagus cuniculus oxaloacetate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2 ferricyanide and 2,6-dichlorophenolindophenol can also act as electron acceptors instead of O2 Oryctolagus cuniculus 2-oxoglutarate + NH3 + H2O2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 optimum depends on the order of addition of the reactands Oryctolagus cuniculus
9.5
-
-
 Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 11 largely destroyed below or over this range Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 fairly stable Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
 D-malate
-
 Oryctolagus cuniculus
0.06
-
 malonate
-
 Oryctolagus cuniculus