KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
O2 | at 4°C | Bos taurus | |
2.2 | - |
D-Aspartate | at 4°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Bos taurus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
recombinant protein, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-aspartate + H2O + O2 | Bos taurus | - |
oxaloacetate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expressed in Escherichia coli, mixture of 80-91% active FAD-containing and 9-20% inactive OH-FAD containing enzyme | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-aspartate + H2O + O2 | - |
Bos taurus | oxaloacetate + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | - |
Bos taurus | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-thiazolidine-2-carboxylate + H2O + O2 | - |
Bos taurus | (ethylthio)oxoacetic acid + H2O2 + NH3 | - |
? | |
additional information | chemical modification with phenylglyoxal results in irreversible loss of activity towards dicarboxylic D-amino acids, paralleled with a transient appearance of activity versus monocarboxylic ones | Bos taurus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
D-Aspartate | 50 mM potassium phosphate buffer, 0.3 mM EDTA, pH 7.4, 4°C | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol flavin per mol protein | Bos taurus |