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Literature summary for 1.4.1.9 extracted from
- Isolation and characterization of leucine dehydrogenase from a thermophilic Citrobacter freundii JK-91 strain isolated from Jask Port (2013), Iran. J. Microbiol., 5, 278-284 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA, Ca2+, Mg2+ | Citrobacter freundii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
L-leucine | pH not specified in the publication, temperature not specified in the publication | Citrobacter freundii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 320000 | - |
gel filtration | Citrobacter freundii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
| Citrobacter freundii JK-91 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 320 | - |
pH not specified in the publication, temperature not specified in the publication | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucine + H2O + NAD+ | - |
Citrobacter freundii | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
| L-leucine + H2O + NAD+ | - |
Citrobacter freundii JK-91 | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
| additional information | no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine | Citrobacter freundii | ? | - |
? | |
| additional information | no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine | Citrobacter freundii JK-91 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 40000, SDS-PAGE | Citrobacter freundii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Citrobacter freundii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 75 | - |
Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Citrobacter freundii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 11 | - |
Citrobacter freundii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor: NADP+, NADPH | Citrobacter freundii | |
| NAD+ | - |
Citrobacter freundii | |
| NADH | - |
Citrobacter freundii | |
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Release 2023.1 (January 2023)
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