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Literature summary for 1.4.1.9 extracted from

  • Ohshima, T.; Nishida, N.; Bakthavatsalam, S.; Kataoka, K.; Takada, H.; Yoshimura, T.; Esaki, N.; Soda, K.
    The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius (1994), Eur. J. Biochem., 222, 305-312.
    View publication on PubMed
Search for more literature for 1.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoactinomyces intermedius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 NADH
-
 Thermoactinomyces intermedius
0.36
-
 NAD+
-
 Thermoactinomyces intermedius
0.4
-
 L-Ile
-
 Thermoactinomyces intermedius
0.63
-
 4-methyl-2-oxopentanoate
-
 Thermoactinomyces intermedius
0.91
-
 2-oxopentanoate
-
 Thermoactinomyces intermedius
1.4
-
 2-Oxohexanoate
-
 Thermoactinomyces intermedius
2
-
 L-Leu
-
 Thermoactinomyces intermedius
2.2
-
 2-oxo-3-methylpentanoate
-
 Thermoactinomyces intermedius
2.4
-
 L-Val
-
 Thermoactinomyces intermedius
2.7
-
 4-methyl-2-oxo-5,5,5-trifluoropentanoate
-
 Thermoactinomyces intermedius
2.8
-
 NADP+ mutant enzyme D203A/I204R Thermoactinomyces intermedius
4.4
-
 3-methyl-2-oxobutanoate
-
 Thermoactinomyces intermedius
7.2
-
 2-oxobutanoate
-
 Thermoactinomyces intermedius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, SDS-PAGE Thermoactinomyces intermedius
340000
-
 gel filtration Thermoactinomyces intermedius

Organism

Organism UniProt Comment Textmining
Thermoactinomyces intermedius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoactinomyces intermedius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
102
-
-
 Thermoactinomyces intermedius
122
-
 recombinant enzyme Thermoactinomyces intermedius

Storage Stability

Storage Stability Organism
4°C, stable for over two years Thermoactinomyces intermedius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxohexanoate + NH3 + NADH + H+ 46% activity compared to 2-oxoisocaproate Thermoactinomyces intermedius L-norleucine + H2O + NAD+
-
 r
4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH
-
 Thermoactinomyces intermedius 2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O
-
 ?
L-2-aminobutanoate + H2O + NAD+
-
 Thermoactinomyces intermedius 2-oxobutanoate + NH3 + NADH + H+
-
 ?
L-2-aminobutanoate + H2O + NAD+
-
 Thermoactinomyces intermedius 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-Ile + H2O + NAD+
-
 Thermoactinomyces intermedius 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Thermoactinomyces intermedius 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Val + H2O + NAD+
-
 Thermoactinomyces intermedius 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Subunits

Subunits Comment Organism
octamer 4 * 42000, SDS-PAGE Thermoactinomyces intermedius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 10 min, retains its full activity Thermoactinomyces intermedius
70
-
 40 min, 3 M NaCl, less than 10% loss of activity Thermoactinomyces intermedius
75
-
 10 min, about 75% loss of activity Thermoactinomyces intermedius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 reductive amination of 3-methyl-2-oxobutanoate Thermoactinomyces intermedius
9.3
-
 reductive amination of 2-oxo-3-methylpentanoate Thermoactinomyces intermedius
9.6
-
 reductive amination of 4-methyl-2-oxopentanoate Thermoactinomyces intermedius
10
-
 deamination of L-Val Thermoactinomyces intermedius
10.5
-
 deamination of L-Leu Thermoactinomyces intermedius

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10.9 50°C, 5 min, stable Thermoactinomyces intermedius
7.5
-
 70°C, 5 min, most stable at pH 7.5 Thermoactinomyces intermedius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermoactinomyces intermedius
NADH
-
 Thermoactinomyces intermedius