Application | Comment | Organism |
---|---|---|
analysis | flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Bacillus sp. (in: Bacteria) | |
Cu2+ | - |
Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 5 mM tetrasodium EDTA solution in phosphate buffer, 0.1 M, pH 7.0, stable for at least 2 months | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu + H2O + NAD+ | - |
Bacillus cereus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus sp. (in: Bacteria) | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 52 | 30°C: about 45% of maximal activity, 48-52°C: maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
denaturation above | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
oxidative deamination of L-Leu | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | 11.5 | pH 10.0: about 40% of maximal activity, pH 11.5: about 80% of maximal activity, oxidative deamination of L-Leu | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus cereus | |
NAD+ | - |
Bacillus sp. (in: Bacteria) | |
NADH | - |
Bacillus cereus | |
NADH | - |
Bacillus sp. (in: Bacteria) |