KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
NADPH | shoots | Pisum sativum | |
0.235 | - |
NADP+ | shoots | Pisum sativum | |
0.538 | - |
2-oxoisovalerate | shoots | Pisum sativum | |
1.1 | - |
L-valine | shoots | Pisum sativum | |
15.5 | - |
NH4+ | shoots | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | sprout | Pisum sativum | - |
- |
soluble | roots | Pisum sativum | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
21fold purification | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sprout | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + H2O + NAD+ | - |
Pisum sativum | 3-methyl-2-oxopentanoate + NADH + NH3 | 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate | ? | |
L-isoleucine + H2O + NAD+ | - |
Pisum sativum | 3-methyl-2-oxopentanoate + NADH + NH3 | reductive amination: 59% of the activity of 2-oxoisovalerate, but no oxidative deamination of L-isoleucine | ? | |
L-valine + NAD(P)+ + H2O | NADP+ | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | NADP+ | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1 | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1 | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | rate of reductive amination of ketoanalogue of valine 5 times greater than rate of oxidative deamination of L-valine | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | NADPH | r | |
L-valine + NAD(P)+ + H2O | rate of reductive amination of ketoanalogue of valine 5 times greater than rate of oxidative deamination of L-valine | Pisum sativum | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
additional information | no oxidative deamination of L-isoleucine and L-alanine | Pisum sativum | additional information | no reductive amination of 2-oxobutyrate | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
oxidative deamination of L-valine | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | in plants enzyme is coupled exclusively to NADP+ | Pisum sativum | |
NADPH | in plants valine dehydrogenase coupled exclusively to NADPH | Pisum sativum |