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Literature summary for 1.4.1.8 extracted from

  • Navarrete, R.M.; Vara, J.A.; Hutchinson, C.R.
    Purification of an inducible L-valine dehydrogenase of Streptomyces coelicolor A3(2) (1990), J. Gen. Microbiol., 136, 273-281.
    View publication on PubMed

General Stability

General Stability Organism
glycerol stabilizes Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
Streptomyces coelicolor
L-valine substrate inhibition at higher concentrations than 20 mM Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
NADH
-
Streptomyces coelicolor
0.17
-
NAD+
-
Streptomyces coelicolor
0.6
-
2-oxoisovalerate
-
Streptomyces coelicolor
10
-
L-valine
-
Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Streptomyces coelicolor
70000
-
gel filtration Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-valine + NAD(P)+ + H2O Streptomyces coelicolor metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
?
L-valine + NAD(P)+ + H2O Streptomyces coelicolor first step in valine catabolism 2-oxoisovalerate + NH3 + NAD(P)H
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
143fold purification Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.7
-
L-alanine Streptomyces coelicolor
1.2
-
L-leucine Streptomyces coelicolor
3.8
-
L-isoleucine Streptomyces coelicolor
14
-
L-valine Streptomyces coelicolor
19
-
L-2-aminobutyrate Streptomyces coelicolor

Storage Stability

Storage Stability Organism
-80Ā°C, 50 mM potassium phosphate pH 7.5, 10% v/v glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1 month, loss of specific activity Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate + H2O + NAD+ 130% of the activity with L-valine Streptomyces coelicolor 2-oxobutyrate + NADH + NH3
-
?
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces coelicolor 2-oxobutyrate + NADH + NH3
-
?
L-alanine + H2O + NAD+ 5% of the activity with L-valine Streptomyces coelicolor pyruvate + NH3 + NADH
-
r
L-cysteine + H2O + NAD+ 3% of the activity with L-valine Streptomyces coelicolor 3-mercapto-2-oxopropanoate + NH3 + NADH
-
?
L-isoleucine + H2O + NAD+ 28% of the activity with L-valine Streptomyces coelicolor 3-methyl-2-oxopentanoate + NADH + NH3
-
?
L-leucine + H2O + NAD+ 8% of the activity with L-valine Streptomyces coelicolor 2-oxoisocaproate + NADH + NH3
-
?
L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H
-
?
L-valine + NAD(P)+ + H2O first step in valine catabolism Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H
-
?
L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
additional information not: D-valine Streptomyces coelicolor additional information
-
?
additional information not: D-valine Streptomyces coelicolor A3(2) additional information
-
?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
reductive amination of 2-oxoisovalerate Streptomyces coelicolor
10.5
-
oxidative deamination of L-valine Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5 pH 8.5: 40% loss of activity, pH 9.5: 10% loss of activity Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
additional information not with NADP+ and NADPH Streptomyces coelicolor
NAD+ NAD+ required, no use of NADP+ Streptomyces coelicolor
NADH NADH could not be replaced with NADPH for the reductive amination Streptomyces coelicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
36.8
-
L-valine
-
Streptomyces coelicolor