Literature summary for 1.4.1.4 extracted from

Pathan, E.K.; Ghormade, V.; Panwar, S.L.; Prasad, R.; Deshpande, M.V.
Molecular studies of NAD- and NADP-glutamate dehydrogenases decipher the conundrum of yeast-hypha dimorphism in zygomycete Benjaminiella poitrasii (2019), FEMS Yeast Res., 19, foz074 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene gdh1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata, expression of BpNADPGDH I does not show any change in morphology of Candida glabrata	Benjaminiella poitrasii
gene gdh3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata, the transformation with hyphal form specific BpNADPGDH II gene leads to germ tube formation in Candida glabrata under normal growth conditions	Benjaminiella poitrasii

Cloned (Comment)

Organism

gene gdh1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata, expression of BpNADPGDH I does not show any change in morphology of Candida glabrata

Benjaminiella poitrasii

gene gdh3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata, the transformation with hyphal form specific BpNADPGDH II gene leads to germ tube formation in Candida glabrata under normal growth conditions

Benjaminiella poitrasii

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a BpNADPGDH II deletion mutant of Benjaminiella poitrasii (DELTAnadpgdh II::HygR) via Agrobacterium-mediated transformation of the BpNADPGDH II disruption cassette	Benjaminiella poitrasii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamate + H2O + NADP+	Benjaminiella poitrasii	-	2-oxoglutarate + NH3 + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Benjaminiella poitrasii	A0A385P597	-	-
Benjaminiella poitrasii	A0A385P5J9	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hypha	-	Benjaminiella poitrasii	-
additional information	analysis of the change in NADP-GDH activity during Y-H transition, overview	Benjaminiella poitrasii	-
additional information	analysis of the changein NADP-GDH activity during Y-H transition, overview	Benjaminiella poitrasii	-
yeast form	-	Benjaminiella poitrasii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + H2O + NADP+	-	Benjaminiella poitrasii	2-oxoglutarate + NH3 + NADPH + H+	-	r

Synonyms

Synonyms	Comment	Organism
BpNADPGDH I	-	Benjaminiella poitrasii
BpNADPGDH II	-	Benjaminiella poitrasii
GDH1	-	Benjaminiella poitrasii
GDH3	-	Benjaminiella poitrasii
H-form specific BpNADPGDH II	-	Benjaminiella poitrasii
NADP-GDH	-	Benjaminiella poitrasii
NADP-glutamate dehydrogenase	-	Benjaminiella poitrasii
Y-form specific BpNADPGDH I	-	Benjaminiella poitrasii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Benjaminiella poitrasii

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Benjaminiella poitrasii
NADPH	-	Benjaminiella poitrasii

General Information

General Information	Comment	Organism
evolution	the BpNADPGDH I and II sequences from Benjaminiella poitrasii include the three typical motifs of the family I hexameric GDHs: 84 PSVNL88, 92KFLGFEQ98 and 184RPEATGY/F 190. One of the conserved regions in the sequences includes the putative NADP-binding motif GSGNVAQYAALKVIELG, located between the residues 219 and 235. BpNADPGDH I and BpNADPGDH II share 70% homology with each other, and BpNADPGDH I and BpNADPGDH II give over 70% identity scores with fungal NADP-dependent GDHs	Benjaminiella poitrasii