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Literature summary for 1.4.1.4 extracted from

  • Du, C.Q.; Lin, J.Z.; Dong, L.A.; Liu, C.; Tang, D.Y.; Yan, L.; Chen, M.D.; Liu, S.; Liu, X.M.
    Overexpression of an NADP(H)-dependent glutamate dehydrogenase gene, TrGDH, from Trichurus improves nitrogen assimilation, growth status and grain weight per plant in rice (2019), Breed. Sci., 69, 429-438 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.4

Application

Application Comment Organism
agriculture enzyme TrGDH is a promising candidate gene for maintaining or improving yields in crop plants via genetic engineering Trichurus sp.
biotechnology enzyme TrGDH is a promising candidate gene for maintaining or improving yields in crop plants via genetic engineering Trichurus sp.

Cloned(Commentary)

Cloned (Comment) Organism
TrGDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant FLAG-tagged enzyme expression in Oryza sativa cv. Kitaake under control of the 35S CaMV 35S promoter, semiquantitative RT-PCR and quantitative real-time PCR enzyme expression analysis Trichurus sp.

Protein Variants

Protein Variants Comment Organism
additional information overexpression of an NADP(H)-dependent glutamate dehydrogenase gene, TrGDH, from Trichurus sp. improves nitrogen assimilation, growth status, and grain weight per plant in rice, phenotype, overview. Compared with the rice GDH (OsGDH4), TrGDH exhibits higher affinity for NH4+ Trichurus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Lineweaver-Burk kinetics Trichurus sp.
1.48
-
 NH3 pH 8.0, 25°C, recombinant enzyme Trichurus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NADP+ Trichurus sp.
-
 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Trichurus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
 Trichurus sp. 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
NADP(H)-dependent glutamate dehydrogenase
-
 Trichurus sp.
NADP(H)-GDH
-
 Trichurus sp.
TrGDH
-
 Trichurus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at, reductive amination reaction Trichurus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at, reductive amination reaction Trichurus sp.

Cofactor

Cofactor Comment Organism Structure
NADP+ beta-NADP+ Trichurus sp.
NADPH beta-NADPH Trichurus sp.