Cloned (Comment) | Organism |
---|---|
genes GDH1 and GDH3, recombinant expression of FLAG-tagged wild-type and mutant GDH1 and GDH3 in yeast cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
K419A | site-directed mutagenesis of GDH1 | Saccharomyces cerevisiae |
K420A | site-directed mutagenesis of GDH1 | Saccharomyces cerevisiae |
K423A | site-directed mutagenesis of GDH1 | Saccharomyces cerevisiae |
K426A | site-directed mutagenesis of GDH1 | Saccharomyces cerevisiae |
additional information | promoter swapping and site-directed mutagenesis of GDH1 and GDH3, construction of gene disruption null mutants of both genes, phenotypes and mutant activities, overview | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | Saccharomyces cerevisiae | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Saccharomyces cerevisiae BY4741 | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
two NADP+-dependent glutamate dehydrogenases encoded by GDH1 and GDH3 | - |
Saccharomyces cerevisiae BY4741 | - |
two NADP+-dependent glutamate dehydrogenases encoded by GDH1 and GDH3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Saccharomyces cerevisiae | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Saccharomyces cerevisiae BY4741 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP+-dependent glutamate dehydrogenase | - |
Saccharomyces cerevisiae |
NADP-GDH | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharomyces cerevisiae | |
NADPH | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | Gdh3-null cells show accelerated chronological aging and hypersusceptibility to thermal and oxidative stress during stationary phase. Upon exposure to oxidative stress, Gdh3-null strains display a rapid loss in viability associated with typical apoptotic hallmarks, i.e. reactive oxygen species accumulation, nuclear fragmentation, DNA breakage, and phosphatidylserine translocation. In addition, Gdh3-null cells, but not Gdh1-null cells, have a higher tendency toward GSH depletion and subsequent reactive oxygen species accumulation than did wild-type cells. GSH depletion is rescued by exogenous GSH or glutamate. The hypersusceptibility of stationary phase Gdh3-null cells to stress-induced apoptosis is suppressed by deletion of GDH2. Gdh1, but not Gdh3, is subjected to stationary phase-specific degradation in which the Lys-426 residue in the Box420Gdh1 region plays an essential role | Saccharomyces cerevisiae |
physiological function | involvement of GDH3-encoded NADP+-dependent glutamate dehydrogenase in yeast cell resistance to stress-induced apoptosis in stationary phase cells, overview. GDH1, but not GDH3, is responsible for the resistance against stress-induced apoptosis in logarithmic phase cells, Necessity of GDH3 for the resistance to stress-induced apoptosis and chronological aging is due to the stationary phase-specific expression of GDH3 and concurrent degradation of Gdh1 in which the Lys-426 residue plays an essential role | Saccharomyces cerevisiae |