Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TG1 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 2-oxoglutarate and NH3 show substrate inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
2-oxoglutarate | in the presence of 100 mM NH4Cl, at 25°C and pH 8 | Escherichia coli | |
1.1 | - |
2-oxoglutarate | in the presence of 200 mM NH4Cl, at 25°C and pH 8 | Escherichia coli | |
1.89 | - |
NH3 | in the presence of 20 mM 2-oxoglutarate, at 25°C and pH 8 | Escherichia coli | |
2.3 | - |
L-glutamate | at 25°C and pH 8 | Escherichia coli | |
2.47 | - |
NH3 | in the presence of 5 mM 2-oxoglutarate, at 25°C and pH 8 | Escherichia coli | |
2.5 | 3 | NH3 | in the presence of 2.5 mM 2-oxoglutarate, at 25°C and pH 8 | Escherichia coli | |
2.5 | 3 | 2-oxoglutarate | in the presence of 400 mM NH4Cl, at 25°C and pH 8 | Escherichia coli | |
18.4 | - |
NADP+ | at 25°C and pH 8 | Escherichia coli | |
59.7 | - |
NADPH | at 25°C and pH 8 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Procion Red HE-3B Sepharose CL-6B column chromatography and Q-Sepharose column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
unpurified supernatant, at 25°C in 0.1 M potassium phosphate containing 100 mM L-glutamate and 0.5 mM NADP+ at pH 8 | Escherichia coli |
36.5 | - |
after 7.4fold purification, at 25°C in 0.1 M potassium phosphate containing 100 mM L-glutamate and 0.5 mM NADP+ at pH 8 | Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, at the low stock enzyme dilutions necessary for kinetic assays GDH loses activity over the duration of an experiment, at 0.01 and 0.02 mg/ml GDH retains 90-95% activity over 24 h in glass tubes, whereas this declines with decreasing storage concentration (to 87%, 71%, and 47% at concentrations of GDH of 5, 2 and 0.5 mg/ml, respectively) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Escherichia coli | 2-oxoglutarate + NH3 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADP+-dependent GDH | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
for reductive amination | Escherichia coli |
9 | - |
for oxidative deamination | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |