Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.4 extracted from

  • Sharkey, M.A.; Engel, P.C.
    Apparent negative co-operativity and substrate inhibition in overexpressed glutamate dehydrogenase from Escherichia coli (2008), FEMS Microbiol. Lett., 281, 132-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain TG1 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information 2-oxoglutarate and NH3 show substrate inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
2-oxoglutarate in the presence of 100 mM NH4Cl, at 25°C and pH 8 Escherichia coli
1.1
-
2-oxoglutarate in the presence of 200 mM NH4Cl, at 25°C and pH 8 Escherichia coli
1.89
-
NH3 in the presence of 20 mM 2-oxoglutarate, at 25°C and pH 8 Escherichia coli
2.3
-
L-glutamate at 25°C and pH 8 Escherichia coli
2.47
-
NH3 in the presence of 5 mM 2-oxoglutarate, at 25°C and pH 8 Escherichia coli
2.5 3 NH3 in the presence of 2.5 mM 2-oxoglutarate, at 25°C and pH 8 Escherichia coli
2.5 3 2-oxoglutarate in the presence of 400 mM NH4Cl, at 25°C and pH 8 Escherichia coli
18.4
-
NADP+ at 25°C and pH 8 Escherichia coli
59.7
-
NADPH at 25°C and pH 8 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Procion Red HE-3B Sepharose CL-6B column chromatography and Q-Sepharose column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
unpurified supernatant, at 25°C in 0.1 M potassium phosphate containing 100 mM L-glutamate and 0.5 mM NADP+ at pH 8 Escherichia coli
36.5
-
after 7.4fold purification, at 25°C in 0.1 M potassium phosphate containing 100 mM L-glutamate and 0.5 mM NADP+ at pH 8 Escherichia coli

Storage Stability

Storage Stability Organism
0°C, at the low stock enzyme dilutions necessary for kinetic assays GDH loses activity over the duration of an experiment, at 0.01 and 0.02 mg/ml GDH retains 90-95% activity over 24 h in glass tubes, whereas this declines with decreasing storage concentration (to 87%, 71%, and 47% at concentrations of GDH of 5, 2 and 0.5 mg/ml, respectively) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
Escherichia coli 2-oxoglutarate + NH3 + NADPH
-
?

Synonyms

Synonyms Comment Organism
NADP+-dependent GDH
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
for reductive amination Escherichia coli
9
-
for oxidative deamination Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli