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Literature summary for 1.4.1.4 extracted from
- Allosteric NADP-glutamate dehydrogenase from aspergilli: purification, characterization and implications for metabolic regulation at the carbon-nitrogen interface (2005), Microbiology, 151, 1409-1419.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3,5-pyrazoledicarboxylate | activates at lower 2-oxoglutarate concentration | Aspergillus niger |  |
| 3,5-pyridinedicarboxylate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
| DL-2-hydroxyglutarate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
| Glutarate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
| Itaconate | activates even in the presence of 10 mM 2-oxoglutarate | Aspergillus niger | |
| oxalylglycine | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-pyridinedicarboxylate | - |
Aspergillus niger | |
| 2-Methyleneglutarate | - |
Aspergillus niger | |
| 2-oxoglutarate | shows allosteric properties and a sigmoid response (nH=2.5) towards 2-oxoglutarate saturation | Aspergillus niger | |
| isophthalate | - |
Aspergillus niger | |
| additional information | not inhibited by D-glutamate, L-glutamine or DL-2-hydroxyglutarate | Aspergillus niger | |
| NADP+ | inhibits at higher 2-oxoglutarate levels | Aspergillus niger | |
| NH4+ | - |
Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NADP+ | - |
Aspergillus niger | |
| 1.05 | - |
NH4+ | - |
Aspergillus niger | |
| 4.78 | - |
2-oxoglutarate | - |
Aspergillus niger | |
| 34.7 | - |
L-glutamate | - |
Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Aspergillus niger |
| 350000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus awamori NCIM 1225 | - |
- |
- |
| Aspergillus nidulans | - |
- |
- |
| Aspergillus nidulans NCIM 1211 | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus niger NCIM 565 | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Aspergillus oryzae NCIM 553 | - |
- |
- |
| Aspergillus terreus | - |
- |
- |
| Aspergillus terreus NCIM 656 | - |
- |
- |
| Saccharomyces cerevisiae | - |
strain Y05499, strain Y10345 and strain Y11672 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, 17fold purified | Aspergillus nidulans |
| by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, more than 7fold purified to homogeneity | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus nidulans | - |
| mycelium | - |
Aspergillus niger | - |
| mycelium | - |
Aspergillus oryzae | - |
| mycelium | - |
Aspergillus terreus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.37 | - |
crude extract | Aspergillus nidulans |
| 2.17 | - |
crude extract | Aspergillus niger |
| 7 | - |
purified enzyme | Aspergillus nidulans |
| 59.4 | - |
purified enzyme | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 week | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus nidulans | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus niger | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus oryzae | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus terreus | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus awamori | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus oryzae NCIM 553 | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus nidulans NCIM 1211 | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus niger NCIM 565 | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus awamori NCIM 1225 | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus terreus NCIM 656 | L-glutamate + NADP+ + H2O | - |
r | |
| 2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger | L-glutamate + NADP+ | - |
? | |
| 2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger NCIM 565 | L-glutamate + NADP+ | - |
? | |
| additional information | no reaction with D-glutamate, L-glutamine or DL-2-hydroxyglutarate as possible substrates in place of L-glutamate, methylamine is unable to replace ammonium in the biosynthetic reaction | Aspergillus niger | ? | - |
? | |
| additional information | no reaction with D-glutamate, L-glutamine or DL-2-hydroxyglutarate as possible substrates in place of L-glutamate, methylamine is unable to replace ammonium in the biosynthetic reaction | Aspergillus niger NCIM 565 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | x * 50000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-GDH | - |
Saccharomyces cerevisiae |
| NADP-GDH | - |
Aspergillus nidulans |
| NADP-GDH | - |
Aspergillus niger |
| NADP-GDH | - |
Aspergillus oryzae |
| NADP-GDH | - |
Aspergillus terreus |
| NADP-GDH | - |
Aspergillus awamori |
| NADP-glutamate dehydrogenase | - |
Saccharomyces cerevisiae |
| NADP-glutamate dehydrogenase | - |
Aspergillus nidulans |
| NADP-glutamate dehydrogenase | - |
Aspergillus niger |
| NADP-glutamate dehydrogenase | - |
Aspergillus oryzae |
| NADP-glutamate dehydrogenase | - |
Aspergillus terreus |
| NADP-glutamate dehydrogenase | - |
Aspergillus awamori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
reductive amination reaction | Aspergillus niger |
| 9.75 | - |
oxidative deamination reaction | Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Aspergillus nidulans | |
| NADP+ | - |
Aspergillus oryzae | |
| NADP+ | - |
Aspergillus terreus | |
| NADP+ | - |
Aspergillus awamori | |
| NADP+ | stimulates the enzyme at lower 2-oxoglutarate levels | Aspergillus niger | |
| NADPH | - |
Aspergillus nidulans | |
| NADPH | - |
Aspergillus niger | |
| NADPH | - |
Aspergillus oryzae | |
| NADPH | - |
Aspergillus terreus | |
| NADPH | - |
Aspergillus awamori | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
NH4+ | - |
Aspergillus niger | |
| 6.9 | - |
isophthalate | - |
Aspergillus niger | |
| 9.2 | - |
2-Methyleneglutarate | - |
Aspergillus niger | |
| 202 | - |
2,4-pyridinedicarboxylate | - |
Aspergillus niger | |
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