Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Benjaminiella poitrasii | the yeast form specific isoenzyme is induced in presence of glucose, the mycelium-form is not induced. Possible involvement of the enzyme in yeast-mycelium transition | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Benjaminiella poitrasii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation increases the activity of the mycelial form of the enzyme. The yeast enzyme remains unaffected. The yeast-form specific native NADP-GDH is in active phosphorylated form. In mycelium the native enzyme is in dephosphorylated inactive form | Benjaminiella poitrasii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | two different enzymes in yeast and in mycelium cells | Benjaminiella poitrasii | - |
yeast form | two different enzymes in yeast and in mycelium cells | Benjaminiella poitrasii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADPH + NH3 | - |
Benjaminiella poitrasii | L-glutamate + NADP+ + H2O | - |
? | |
additional information | the yeast form specific isoenzyme is induced in presence of glucose, the mycelium-form is not induced. Possible involvement of the enzyme in yeast-mycelium transition | Benjaminiella poitrasii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADP-dependent glutamate dehydrogenase | 2 isoenzymes: a yeast-form isoenzyme and a mycelium-form isoenzyme | Benjaminiella poitrasii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
enzyme from mycelium | Benjaminiella poitrasii |
32 | - |
enzyme from yeast | Benjaminiella poitrasii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
60 min, yeast enzyme retains 41% of its activity, mycelial enzyme retains 9% of its activity | Benjaminiella poitrasii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzyme from mycelium and yeast | Benjaminiella poitrasii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Benjaminiella poitrasii |