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Literature summary for 1.4.1.4 extracted from
- Purification and some properties of the glutamate dehydrogenase of Salmonella typhimurium (1973), Can. J. Microbiol., 19, 427-438.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoic acid | at 10 mM, 90% inhibition after 40 min | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| sodium dodecylsulfate | at 0.7% w/v after 1 h 5% activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 280000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
| 291000 | - |
sucrose-density-gradient centrifugation | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min stable | Salmonella enterica subsp. enterica serovar Typhimurium |
| 65 | - |
complete inactivation | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
reductive amination | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
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