Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
E158Q | 3.3% as active as wild-type | Thermococcus kodakarensis |
T138E | 1.6% as active as wild-type | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47300 | - |
alpha6, 6 * 47300, SDS-PAGE | Thermococcus kodakarensis |
284000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Thermococcus kodakarensis | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
T138E-mutant | Thermococcus kodakarensis |
80 | - |
E158Q-mutant | Thermococcus kodakarensis |
2500 | - |
wild-type | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | - |
Thermococcus kodakarensis | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 47300, SDS-PAGE | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
E158Q-mutant | Thermococcus kodakarensis |
85 | - |
T138E-mutant | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
wild-type and T138E 50% activity after 2 h and 3 h respectively | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermococcus kodakarensis | |
NADPH | - |
Thermococcus kodakarensis |