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Literature summary for 1.4.1.4 extracted from

  • Ma, K.; Robb, F.T.; Adams, M.W.
    Purification and characterization of NADP-specific alcohol dehydrogenase and glutamate dehydrogenase from the hyperthermophilic archaeon Thermococcus litoralis (1994), Appl. Environ. Microbiol., 60, 562-568.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
NADP+ oxidative deamination at 80°C Thermococcus litoralis
0.14
-
NADPH reductive amination at 80°C Thermococcus litoralis
0.16
-
2-oxoglutarate reductive amination at 80°C Thermococcus litoralis
0.22
-
L-glutamate oxidative deamination at 80°C Thermococcus litoralis
0.63
-
NH4+ reductive amination at 80°C Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
alpha6, 6 * 45000, SDS-PAGE Thermococcus litoralis
350000
-
gel filtration Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Thermococcus litoralis
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
oxidative deamination at 80°C and pH 8.0 Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Thermococcus litoralis L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Thermococcus litoralis 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 45000, SDS-PAGE Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
oxidative deamination Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
no activity loss after 5 h Thermococcus litoralis
98
-
50% activity after 2 h Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
oxidative deamination at 80°C Thermococcus litoralis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Thermococcus litoralis
NADPH
-
Thermococcus litoralis