KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
NADP+ | oxidative deamination at 80°C | Thermococcus litoralis | |
0.14 | - |
NADPH | reductive amination at 80°C | Thermococcus litoralis | |
0.16 | - |
2-oxoglutarate | reductive amination at 80°C | Thermococcus litoralis | |
0.22 | - |
L-glutamate | oxidative deamination at 80°C | Thermococcus litoralis | |
0.63 | - |
NH4+ | reductive amination at 80°C | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
alpha6, 6 * 45000, SDS-PAGE | Thermococcus litoralis |
350000 | - |
gel filtration | Thermococcus litoralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Thermococcus litoralis | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
oxidative deamination at 80°C and pH 8.0 | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Thermococcus litoralis | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Thermococcus litoralis | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 45000, SDS-PAGE | Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
oxidative deamination | Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
no activity loss after 5 h | Thermococcus litoralis |
98 | - |
50% activity after 2 h | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
oxidative deamination at 80°C | Thermococcus litoralis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermococcus litoralis | |
NADPH | - |
Thermococcus litoralis |