Cloned (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
- |
Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 1.3fold increase in activity at 10 mM CaCl2 or MgSO4 | Thermococcus litoralis | |
Ca2+ | addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 3.5fold increase in activity at 10 mM CaCl2 or MgSO4 | Pyrococcus furiosus | |
Mg2+ | addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 1.3fold increase in activity at 10 mM CaCl2 or MgSO4 | Thermococcus litoralis | |
Mg2+ | addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 3.5fold increase in activity at 10 mM CaCl2 or MgSO4 | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P80319 | - |
- |
Thermococcus litoralis | Q56304 | - |
- |
Thermococcus litoralis DSM 5473 | Q56304 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Thermococcus litoralis | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? | |
L-glutamate + H2O + NADP+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? | |
L-glutamate + H2O + NADP+ | - |
Thermococcus litoralis DSM 5473 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
97 | - |
specific activity increases up to 97°C | Thermococcus litoralis |
97 | - |
specific activity increases up to 97°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
104 | - |
half-life: 4.9 min in absence of KCl, 45 min in presence of 1 M KCl | Thermococcus litoralis |
104 | - |
half-life: 67 min in absence of KCl, 562 min in presence of 1 M KCl | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Thermococcus litoralis |
8 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity | Thermococcus litoralis |
7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 35% of maximal activity | Pyrococcus furiosus |