Literature summary for 1.4.1.3 extracted from

Consalvi, V.; Chiaraluce, R.; Politi, L.; Pasquo, A.; De Rosa, M.; Scandurra, R.
Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus: studies on thermal and guanidine-dependent inactivation. (1993), Biochim. Biophys. Acta, 1202, 207-215.

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General Stability

General Stability	Organism
guanidine-dependent inactivation of the enzyme at 20°C is irreversible above 1.5 M guanidine hydrochloride	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, SDS-PAGE	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NADPH + NH3	-	Saccharolobus solfataricus	L-glutamate + NADP+ + H2O	-	r

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
NAD(P)-dependent glutamate dehydrogenase	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	incubation for 1 h at 0.2 mg/ml results in 90% loss of activity, incubation for 24 h at 0.8 mg/ml results in 30% loss of activity. Inactivation is irreversible. 3 M guanidine-HCl increases the half-life of the enzyme at 90°C and 0.2 mg/ml 6fold. Half-life at 90°C and 0.2 mg/ml protein concentration increases more than 6fold in the presence of 0.4 M Na2SO4 and decreases 4fold in the presence of 0.4 M NaSCN	Saccharolobus solfataricus
95	-	half-life: 15 min at 0.4 mg/ml, 30 min at 0.8 mg/ml. Temperature-dependent inactivation of the enzyme is irreversible, this process is accompanied by a progressive increase in hydrophobic surface area which leads to protein precipitation	Saccharolobus solfataricus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.2	6.8	maximal stablility	Saccharolobus solfataricus

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Release 2023.1 (January 2023)