Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | in absence of GTP the isoenzyme GLUD2 assumes a conformational state associated with little catalytic activity, it remains amenable to full activation by ADP and/or L-Leu | Homo sapiens | |
L-Leu | 0.3-10 mM stimulates isoenzyme GLUD2 to a greater extent than isoenzyme GLUD1 | Homo sapiens | |
L-Leu | 0.3-10 mM stimulates isoenzyme GLUD2 to a greater extent than isoenzyme GLUD1. In absence of GTP the isoenzyme GLUD2 assumes a conformational state associated with little catalytic activity, it remains amenable to full activation by ADP and/or L-Leu | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Sf9 cells using the baculovirus expression system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G456A | mutant enzyme is resistant to GTP | Homo sapiens |
R443S | mutation abolishes basal activity and renders the enzyme dependent on ADP for function | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | potent inhibitor. IC50 for wild-type enzyme is 0.00019 mM, IC50 for mutant enzyme G456A is 0.0028 mM. ADP renders the GLUD1-derived enzyme less sensitive to GTP inhibition; totally insensitive to, it becomes amenable to GTP inhibition in presence of ADP | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00367 | - |
- |
Homo sapiens | P49448 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | predominantly | Homo sapiens | - |
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | - |
Homo sapiens | L-glutamate + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLUD1 | wildely expressed isoenzyme | Homo sapiens |
GLUD2 | nerve-tissue-specific isoenzyme | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 25-30% less active at pH 7.0 than at pH 7.5 and 8.0 | Homo sapiens |
7 | 8 | equally active at pH 7.0 and 8.0 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00019 | - |
potent inhibitor. IC50 for wild-type enzyme is 0.00019 mM | Homo sapiens | GTP | |
0.0028 | - |
IC50 for mutant enzyme G456A is 0.0028 mM. ADP renders the GLUD1-derived enzyme less sensitive to GTP inhibition | Homo sapiens | GTP |