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Literature summary for 1.4.1.3 extracted from
- Occurrence of two different glutamate dehydrogenase activities in the halophilic bacterium Salinibacter ruber (2003), FEMS Microbiol. Lett., 226, 181-186.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | activates in presence of 3 M NaCl or 3 M KCl or in absence of salts | Salinibacter ruber |  |
| ATP | activates in presence of 3 M NaCl or in absence of salts, slight inhibition in presence of 3 M KCl | Salinibacter ruber | |
| L-His | activates in presence of 3 M NaCl, 3 M Kcl or in absence of salts | Salinibacter ruber | |
| L-Leu | activates in presence of 3 M NaCl, 3 M Kcl or in absence of salts | Salinibacter ruber | |
General Stability
| General Stability | Organism |
|---|---|
| strong dependence on high salt concentrations for stability | Salinibacter ruber |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
NADH | pH 8.0 | Salinibacter ruber | |
| 0.42 | - |
NADPH | pH 8.0 | Salinibacter ruber | |
| 7.1 | - |
2-oxoglutarate | pH 8.0 | Salinibacter ruber | |
| 68 | - |
NH3 | pH 8.0 | Salinibacter ruber | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Salinibacter ruber | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 3 mM, activates to 144% of control | Salinibacter ruber | |
| NaCl | 3 M, 67fold activation | Salinibacter ruber | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
8 * 61000, or aggregate of two tetramers, SDS-PAGE | Salinibacter ruber |
| 460000 | - |
gel filtration | Salinibacter ruber |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salinibacter ruber | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salinibacter ruber |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salinibacter ruber |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH3 + NADH | - |
Salinibacter ruber | L-glutamate + H2O + NAD+ | - |
r | |
| 2-oxoglutarate + NH3 + NADPH + H+ | - |
Salinibacter ruber | L-glutamate + H2O + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 61000, or aggregate of two tetramers, SDS-PAGE | Salinibacter ruber |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDHII | - |
Salinibacter ruber |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
amination reaction | Salinibacter ruber |
| 8.5 | - |
deamination, in 50 mM Tris-HCl buffer containing 3 M KCl | Salinibacter ruber |
| 9.5 | - |
deamination, in 50 mM CHES buffer (N-cyclohexyl-2-aminoethanesulfonic acid) containing 3 M NaCl | Salinibacter ruber |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Salinibacter ruber | |
| NADPH | at lower maximal rate than with NADH | Salinibacter ruber | |
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