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Literature summary for 1.4.1.3 extracted from

  • Rahman, R.N.Z.A.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymic characteristics of native and recombinant GDHs (1998), Mol. Gen. Genet., 257, 338-347.
    View publication on PubMed
Search for more literature for 1.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NADPH concentration range: 0.002-0.1 mM Pyrococcus
0.08
-
 NADP+ concentration range: 0.02-0.2 mM Pyrococcus
0.09
-
 NADH concentration range: 0.04-0.1 mM Pyrococcus
0.25
-
 2-oxoglutarate concentration range: 0.3-4.0 mM Pyrococcus
0.32
-
 glutamate concentration range: 0.05-1.0 mM Pyrococcus
1.3
-
 NH4+ concentration range: 0.2-5 mM Pyrococcus
5.5
-
 glutamate concentration range: 1.0-10.0 mM Pyrococcus
13
-
 NH4+ concentration range: 10-200 mM Pyrococcus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47300
-
 6 * 47300, recombinant enzyme, second peak in gel filtration corresponding to catalytically inactive monomer, SDS-PAGE Pyrococcus
284000
-
 recombinant and native enzyme, gel filtration Pyrococcus

Organism

Organism UniProt Comment Textmining
Pyrococcus
-
 sp. KOD1, hyperthermophilic archeon isolated from kodakara island, japan
-

Purification (Commentary)

Purification (Comment) Organism
HitrapQ, Hitrap blue, recombinant and native enzyme Pyrococcus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
-
 Pyrococcus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+
-
 Pyrococcus L-glutamate + H2O + NAD+
-
 r
L-glutamate + H2O + NAD(P)+
-
 Pyrococcus 2-oxoglutarate + NH3 + NAD(P)H
-
 r
oxaloacetate + NH3 + NADH + H+
-
 Pyrococcus L-aspartate + H2O + NAD+
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 47300, recombinant enzyme, second peak in gel filtration corresponding to catalytically inactive monomer, SDS-PAGE Pyrococcus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
 glutamate concentration range: 0.05-1.0 mM Pyrococcus
63.3
-
 NH4+ concentration range: 0.2-5 mM Pyrococcus
90
-
 NADH concentration range: 0.04-0.1 mM Pyrococcus
102
-
 NADP+ concentration range: 0.02-0.2 mM Pyrococcus
112
-
 NADPH concentration range: 0.002-0.1 mM Pyrococcus
162
-
 glutamate concentration range: 1.0-10.0 mM Pyrococcus
168
-
 NH4+ concentration range: 10-200 mM Pyrococcus
195
-
 2-oxoglutarate concentration range: 0.3-4.0 mM Pyrococcus

Cofactor

Cofactor Comment Organism Structure
NADPH 3fold higher activity than with NADH Pyrococcus