Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | limited trypsin proteolyis activates the purified enzyme 8fold if the peptide is absent from the assay mixture, the native enzyme is 3fold activated if the cleaved peptide is present, activation may therefore be induced by loss of the peptide from the subunit of the native enzyme | Anguilla anguilla |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
6 * 54000, SDS-PAGE | Anguilla anguilla |
340000 | - |
gel filtration | Anguilla anguilla |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anguilla anguilla | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | limited trypsin proteolysis removes a 39 amino acid peptide from the enzyme | Anguilla anguilla |
Purification (Comment) | Organism |
---|---|
hydroxylapatite, DEAE-Toyopearl, GTP-Sepharose | Anguilla anguilla |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Anguilla anguilla | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.53 | - |
- |
Anguilla anguilla |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Anguilla anguilla | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 54000, SDS-PAGE | Anguilla anguilla |