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Literature summary for 1.4.1.27 extracted from

  • Wittmiss, M.; Mikkat, S.; Hagemann, M.; Bauwe, H.
    Stoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system (2020), Plant J., 103, 801-813 .
    View publication on PubMed
Search for more literature for 1.4.1.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.83
-
 lipoic acid pH 6.3, 25°C Synechocystis sp. PCC 6803

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Pisum sativum 5739
-
mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000 80000 H-protein, gel filtration Synechocystis sp. PCC 6803
150000
-
 L-protein, gel filtration Synechocystis sp. PCC 6803
200000
-
 P-protein, gel filtration Synechocystis sp. PCC 6803
1550000 1650000 minimum mass of the leaf GDC, mass spectrometry Pisum sativum
1550000 1650000 minimum mass of the leaf GDC, mass spectrometry Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M5K3 and Q94B78 and P25855 and Q9LQL0 and O65396 Q9M5K3 i.e. dihydrolipoyl dehydrogenase component L-protein, cf. EC 1.8.1.4, Q94B78 i.e. glycine dehydrogenase component P-protein, cf. EC 1.4.4.2, P25855 i.e. component H-protein isoform H1, Q9LQL0 i.e. component H-protein isoform H3, O65396 i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10
-
Pisum sativum P31023 and P26969 and P16048 and P49364 P31023 i.e. dihydrolipoyl dehydrogenase component L-protein, cf. EC 1.8.1.4, P26969 i.e. glycine dehydrogenase component P-protein, cf. EC 1.4.4.2, P16048 i.e. component H-protein, P49364 i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10
-
Synechocystis sp. PCC 6803 P72740 and P54261 P72740 i.e. dihydrolipoyl dehydrogenase component L-protein, cf. EC 1.8.1.4, P54261 i.e. aminomethyltransferase component T-protein, cf. EC 2.1.2.10
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pisum sativum
-
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific maximum activities are 0.37 micromol per min and mg for the P-protein (bicarbonate-exchange reaction) Synechocystis sp. PCC 6803
14
-
 assay of L-protein with lipoic acid, 25°C, pH 6.3 Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + tetrahydrofolate + NAD+
-
 Synechocystis sp. PCC 6803 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
 ?
lipoic acid + NADH + H+ assay of isolated L-protein, cf. EC 1.8.1.4 Synechocystis sp. PCC 6803 dihydrolipoic acid + NAD+
-
 ?

Subunits

Subunits Comment Organism
More the H-isoproteins GCS-H1 and GCS-H3 are fully redundant. Isoform GCS-H2 is not present in leaf mitochondria Arabidopsis thaliana
More the molar ratios of the leaf GDC component proteins are 1* L-protein dimer, 0.5 * P-protein dimer, 0.6 * T-protein and 2.4 * H-protein, determined by mass spectrometry Synechocystis sp. PCC 6803
multimer 2 * 100000, P-protein dimer, SDS-PAGE, 2 * 50832, L-protein dimer Synechocystis sp. PCC 6803
multimer the molar ratios of the leaf GDC component proteins are 1* L-protein dimer, 4 * P-protein dimer, 8 * T-protein and 20 * H-protein, determined by mass spectrometry Arabidopsis thaliana
multimer the molar ratios of the leaf GDC component proteins are 1* L-protein dimer, 4 * P-protein dimer, 8 * T-protein and 26 H-protein, determined by mass spectrometry Pisum sativum

Synonyms

Synonyms Comment Organism
At1g11860 gene name, aminomethyltransferase Arabidopsis thaliana
At1g32470 gene name, H-protein isoform GCS-H1 Arabidopsis thaliana
At1g48030 gene name, dihydrolipoyl dehydrogenase Arabidopsis thaliana
AT2G35370 gene name, H-protein isoform GCS-H1 Arabidopsis thaliana
At4g33010 gene name, glycine dehydrogenase Arabidopsis thaliana
GDCSH H-protein Pisum sativum
GDCSP glycine dehydrogenase Pisum sativum
GDSCT aminomethyltransferase Pisum sativum
sll0171 gene name, aminomethyltransferase Synechocystis sp. PCC 6803
slr1096 gene name, L-protein Synechocystis sp. PCC 6803