KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADH | pH 10.4, 30°C | Streptomyces fradiae | |
0.04 | - |
NAD+ | pH 10.4, 30°C | Streptomyces fradiae | |
0.31 | - |
2-oxoisovalerate | pH 10.4, 30°C | Streptomyces fradiae | |
0.43 | - |
L-valine | pH 10.4, 30°C | Streptomyces fradiae | |
25.6 | - |
NH4+ | pH 10.4, 30°C | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Streptomyces fradiae |
80000 | - |
gel filtration | Streptomyces fradiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces fradiae |
Storage Stability | Organism |
---|---|
-20°C, pH 7.4, presence of 2-mercaptoethanol and 30% glycerol, loss of 90% of activity with 24 h | Streptomyces fradiae |
4°C, pH 7.4, presence of 2-mercaptoethanol and 10% glycerol, stable for 48 h | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | 100% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxoisohexanoate + NH3 + NADH + H+ | 20% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-leucine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH3 + NADH + H+ | - |
Streptomyces fradiae | L-valine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH4+ + NADH | - |
Streptomyces fradiae | L-valine + H2O + NAD+ | - |
r | |
3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 16.6% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-isoleucine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 158% of the activity with L-valine | Streptomyces fradiae | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | 8.3% of the activity with L-valine | Streptomyces fradiae | pyruvate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 22% of the activity with L-valine | Streptomyces fradiae | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 24% of the activity with L-valine | Streptomyces fradiae | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | 34.5% of the activity with L-valine | Streptomyces fradiae | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 96% of the activity with L-valine | Streptomyces fradiae | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Streptomyces fradiae | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
pyruvate + H2O + NAD+ | 23.3% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-alanine + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Streptomyces fradiae |
Synonyms | Comment | Organism |
---|---|---|
VDH2 | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
both oxidative deamination and reductive amination | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
reductive amination | Streptomyces fradiae |
10.4 | - |
oxidative deamination | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1,N6-etheno-NAD+ | 70.5% of the activity with NAD+ | Streptomyces fradiae | |
3-pyridinealdehyde-NAD+ | 47% of the activity with NAD+ | Streptomyces fradiae | |
deamino-NAD+ | 124% of the activity with NAD+ | Streptomyces fradiae | |
additional information | no activity with NADP+ | Streptomyces fradiae | |
NAD+ | - |
Streptomyces fradiae | |
NADH | - |
Streptomyces fradiae |
Organism | Comment | Expression |
---|---|---|
Streptomyces fradiae | activity is repressed in presence of ammonia | down |
Streptomyces fradiae | activity is induced in presence of L-valine | up |