Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | HgCl2: 0.01 mM, 91% loss of activity | Kitasatospora aureofaciens | |
additional information | no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil | Kitasatospora aureofaciens | |
p-chloromercuribenzoate | 0.01 mM, 83% loss of activity | Kitasatospora aureofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NADH | - |
Kitasatospora aureofaciens | |
0.023 | - |
NAD+ | pH 10.4, 30°C | Kitasatospora aureofaciens | |
0.1 | - |
NAD+ | - |
Kitasatospora aureofaciens | |
0.8 | - |
2-oxoisocaproate | - |
Kitasatospora aureofaciens | |
0.8 | - |
2-Oxoisohexanoate | pH 10.4, 30°C | Kitasatospora aureofaciens | |
1.25 | - |
2-oxoisovalerate | - |
Kitasatospora aureofaciens | |
1.3 | - |
2-oxoisovalerate | pH 10.4, 30°C | Kitasatospora aureofaciens | |
1.5 | - |
2-oxobutyrate | - |
Kitasatospora aureofaciens | |
1.5 | - |
2-oxobutanoate | pH 10.4, 30°C | Kitasatospora aureofaciens | |
2.5 | - |
L-valine | - |
Kitasatospora aureofaciens | |
2.5 | - |
L-valine | pH 10.4, 30°C | Kitasatospora aureofaciens | |
5 | - |
L-isoleucine | - |
Kitasatospora aureofaciens | |
5 | - |
L-isoleucine | pH 10.4, 30°C | Kitasatospora aureofaciens | |
5.7 | - |
L-norvaline | - |
Kitasatospora aureofaciens | |
5.7 | - |
L-norvaline | pH 10.4, 30°C | Kitasatospora aureofaciens | |
6.3 | - |
L-leucine | - |
Kitasatospora aureofaciens | |
6.3 | - |
L-leucine | pH 10.4, 30°C | Kitasatospora aureofaciens | |
6.7 | - |
2-oxo-3-methylvalerate | - |
Kitasatospora aureofaciens | |
6.7 | - |
3-methyl-2-oxopentanoate | pH 10.4, 30°C | Kitasatospora aureofaciens | |
14.8 | - |
L-2-aminobutyrate | - |
Kitasatospora aureofaciens | |
14.8 | - |
L-2-aminobutanoate | pH 10.4, 30°C | Kitasatospora aureofaciens | |
15.3 | - |
pyruvate | - |
Kitasatospora aureofaciens | |
15.3 | - |
pyruvate | pH 10.4, 30°C | Kitasatospora aureofaciens | |
15.6 | - |
L-norleucine | - |
Kitasatospora aureofaciens | |
15.6 | - |
L-norleucine | pH 10.4, 30°C | Kitasatospora aureofaciens | |
18.2 | - |
NH4+ | - |
Kitasatospora aureofaciens | |
18.2 | - |
NH3 | pH 10.4, 30°C | Kitasatospora aureofaciens | |
333 | - |
L-alanine | pH 10.4, 30°C | Kitasatospora aureofaciens | |
333.6 | - |
L-alanine | - |
Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
4 * 29000, SDS-PAGE | Kitasatospora aureofaciens |
116000 | - |
equilibrium ultracentrifugation | Kitasatospora aureofaciens |
116000 | 118000 | gel filtration and equilibrium ultracentrifugation | Kitasatospora aureofaciens |
118000 | - |
gel filtration | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
- |
- |
Kitasatospora aureofaciens | - |
a UV mutant derived from the high-producing strain 84/25 | - |
Kitasatospora aureofaciens 50/137 | - |
a UV mutant derived from the high-producing strain 84/25 | - |
Purification (Comment) | Organism |
---|---|
- |
Kitasatospora aureofaciens |
785fold purification | Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Kitasatospora aureofaciens |
Storage Stability | Organism |
---|---|
-25°C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity | Kitasatospora aureofaciens |
4°C, 70% (NH4)2SO4 suspension, 3 weeks, no loss of activity | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | 48.2% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxoisohexanoate + NH3 + NADH + H+ | 38.1% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-leucine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH3 + NADH + H+ | - |
Kitasatospora aureofaciens | L-valine + H2O + NAD+ | - |
r | |
3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 22.3% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-isoleucine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 16.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 16.5% of the activity with L-valine | Kitasatospora aureofaciens 50/137 | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutyrate + H2O + NAD+ | 16.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxobutyrate + NADH + NH3 | reductive amination: 48.2% of the activity with 2-oxoisovalerate | r | |
L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Kitasatospora aureofaciens | 2-oxobutyrate + NADH + NH3 | reductive amination: 48.2% of the activity with 2-oxoisovalerate | r | |
L-alanine + H2O + NAD+ | very poor substrate: 1.2% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH | pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate | r | |
L-alanine + H2O + NAD+ | very poor substrate: 1.2% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH | reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate | r | |
L-alanine + H2O + NAD+ | 1.9% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 46.5% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NADH + NH3 | 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate | r | |
L-isoleucine + H2O + NAD+ | 46.5% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NADH + NH3 | reductive amination: 22.3% of the activity with 2-oxoisovalerate | r | |
L-isoleucine + H2O + NAD+ | 46.5% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 36% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisocaproate + NADH + NH3 | 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate | r | |
L-leucine + H2O + NAD+ | 36% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisocaproate + NADH + NH3 | reductive amination: 38.1% of the activity with 2-oxoisovalerate | r | |
L-leucine + H2O + NAD+ | 36% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | 10.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxocaproate + NADH + NH3 | - |
r | |
L-norleucine + H2O + NAD+ | 10.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 43% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxovalerate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | 43% of the activity with L-valine | Kitasatospora aureofaciens 50/137 | 2-oxovalerate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | 43.0% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Kitasatospora aureofaciens | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | highest activity with 2-oxoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens 50/137 | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens 50/137 | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens 50/137 | 2-oxoisovalerate + NH3 + NAD(P)H | highest activity with 2-oxoisovalerate | r | |
pyruvate + H2O + NAD+ | 1.3% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-alanine + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29000, SDS-PAGE | Kitasatospora aureofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kitasatospora aureofaciens |
65 | - |
- |
Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination | Kitasatospora aureofaciens |
9 | - |
reductive amination of 2-oxoisovalerate, Tris-HCl buffer | Kitasatospora aureofaciens |
10.7 | - |
oxidative deamination | Kitasatospora aureofaciens |
10.7 | - |
oxidative deamination of L-valine, glycine-KCl-KOH buffer | Kitasatospora aureofaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1,N6-etheno-NAD+ | 58.4% of the activity with NAD+ | Kitasatospora aureofaciens | |
3-acetylpyridine-NAD+ | 100% of the activity with NAD+ | Kitasatospora aureofaciens | |
3-pyridinealdehyde-NAD+ | 37.4% of the activity with NAD+ | Kitasatospora aureofaciens | |
deamino-NAD+ | 83.7% of the activity with NAD+ | Kitasatospora aureofaciens | |
additional information | no cofactor: NADP+ | Kitasatospora aureofaciens | |
NAD+ | - |
Kitasatospora aureofaciens | |
NAD+ | NAD+ required, no use of NADP+ | Kitasatospora aureofaciens | |
NADH | - |
Kitasatospora aureofaciens | |
NADP+ | only 8.4% of the activity with NAD+ | Kitasatospora aureofaciens | |
nicotinamide guanine dinucleotide | 72.3% of the activity with NAD+ | Kitasatospora aureofaciens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Kitasatospora aureofaciens | isoelectric focusing | - |
5.1 |