Application | Comment | Organism |
---|---|---|
analysis | development of a genetically encoded fluorescent protein construct for monitoring of L-Asp in vitro, and employment of aspartate dehydrogenase scaffold as a biorecognition element | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expression of N-terminally His-tagged and GFP-tagged enzyme, using the flexible GGSGG linker, in Escherichia coli. The recombinant tagged aspartate dehydrogenase functions as the biorecognition element, and aspartate-induced conformational change is converted to a fluorescence signal by GFP, method, overview | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | Thermotoga maritima | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | - |
Thermotoga maritima | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
additional information | high substrate specificity of aspartate dehydrogenase enzyme | Thermotoga maritima | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermotoga maritima | |
NADH | - |
Thermotoga maritima | |
NADP+ | - |
Thermotoga maritima | |
NADPH | - |
Thermotoga maritima |