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Literature summary for 1.4.1.21 extracted from

  • Okamura, T.; Noda, H.; Fukuda, S.; Ohsugi, M.
    Aspartate dehydrogenase in vitamin B12-producing Klebsiella pneumoniae IFO 13541 (1998), J. Nutr. Sci. Vitaminol., 44, 483-490.
    View publication on PubMed
Search for more literature for 1.4.1.21

General Stability

General Stability Organism
enzyme activity in Tris-HCl buffer is about 7fold higher than in potassium phosphate buffer Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Klebsiella pneumoniae
K+ potassium ion in phosphate buffer may be inhibitory Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 2 * 62000, SDS-PAGE Klebsiella pneumoniae
124000
-
 gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + NADP+ + H2O Klebsiella pneumoniae
-
 oxaloacetate + NH4+ + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 vitamin B12-producing strain IFO 13541
-

Purification (Commentary)

Purification (Comment) Organism
about 500fold Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.045
-
 pH 7, 30°C, crude extract Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + NADP+ + H2O
-
 Klebsiella pneumoniae oxaloacetate + NH4+ + NADPH
-
 ?
additional information not: D-aspartate, L-glutamate, L-glycine, L-alanine, L-threonine, L-serine, L-leucine, L-isoleucine, L-methionine, L-cysteine, L-proline, L-valine, L-phenylalanine, L-tyrosine, L-tryptophan, L-lysine, L-histidine, L-arginine Klebsiella pneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 62000, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
NAD-dependent aspartate dehydrogenase
-
 Klebsiella pneumoniae
NADH2-dependent aspartate dehydrogenase
-
 Klebsiella pneumoniae
NADP+-dependent aspartate dehydrogenase
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 up to 20°C, 10 min, Tris-HCl buffer, pH 7.5, stable Klebsiella pneumoniae
50
-
 10 min, Tris-HCl buffer, pH 7.5, 70% loss of activity Klebsiella pneumoniae
60
-
 10 min, Tris-HCl buffer, pH 7.5, 100% loss of activity Klebsiella pneumoniae
100
-
 boiling inactivates Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in Tris-HCl buffer, oxidative deamination of L-aspartate Klebsiella pneumoniae
8
-
 in potassium phosphate buffer, oxidative deamination of L-aspartate Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
additional information not: NAD+, FMN, FAD Klebsiella pneumoniae
NADP+ requires NADP+ as coenzyme Klebsiella pneumoniae