Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethylene glycolmonomethyl ether | the catalytic efficiency in ethylene glycolmonomethyl ether is 1.15- and 1.35fold than that of in the aqueous phase | Fictibacillus nanhaiensis | |
methanol | the catalytic efficiency in 30% (v/v) methanol is 1.15fold than that of in the aqueous phase | Fictibacillus nanhaiensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Fictibacillus nanhaiensis |
General Stability | Organism |
---|---|
after pre-treatment with 1 M NaCl at 55°C for 1 h, the enzyme exhibits high activity and retains over 50% of its maximal activity during pH 7.0-8.5 | Fictibacillus nanhaiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | in the presence of acetonitrile at 10% (v/v), the enzyme retains about 60% of the original activity. However, the enzyme exhibits nearly no activity in the presence of acetonitrile at 30% (v/v) | Fictibacillus nanhaiensis | |
Isopropanol | in the presence of isopropanol at 10% (v/v), the enzyme retains about 30% of the original activity. However, the enzyme exhibits nearly no activity in the presence of isopropanol at 30% (v/v) | Fictibacillus nanhaiensis | |
additional information | the enzyme exhibits nearly no change on enzyme activity in the presence of glycol, ethanol and methanol at 10% (v/v) | Fictibacillus nanhaiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | salt ions have little effect on enzyme activity | Fictibacillus nanhaiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | Fictibacillus nanhaiensis | - |
phenylpyruvate + NH3 + NADH + H+ | - |
? | |
L-phenylalanine + H2O + NAD+ | Fictibacillus nanhaiensis DSF-15A2 | - |
phenylpyruvate + NH3 + NADH + H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | the enzyme shows high activity in the presence of acetone from 10% (v/v) to 30% (v/v), retaining 100% of the original activity | Fictibacillus nanhaiensis |
ethylene glycol monomethyl ether | the enzyme shows high activity in the presence of ethylene glycol monomethyl ether from 10% (v/v) to 30% (v/v), retaining 100% of the original activity | Fictibacillus nanhaiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fictibacillus nanhaiensis | A0A1B1PGG4 | - |
- |
Fictibacillus nanhaiensis DSF-15A2 | A0A1B1PGG4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-IDA column chromatography | Fictibacillus nanhaiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | - |
Fictibacillus nanhaiensis | phenylpyruvate + NH3 + NADH + H+ | - |
? | |
L-phenylalanine + H2O + NAD+ | - |
Fictibacillus nanhaiensis DSF-15A2 | phenylpyruvate + NH3 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x 40710, His-tagged enzyme, calculated from amino acid sequence | Fictibacillus nanhaiensis |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Fictibacillus nanhaiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Fictibacillus nanhaiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | at temperatures from 0 to 60°C, the enzyme retains over 70% of its maximal activity at 40°C | Fictibacillus nanhaiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | after incubation at 30-55°C for 1 h under pH 10.0, the enzyme retains over 60% of its maximal activity while it retains about no activity at 55°C | Fictibacillus nanhaiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Fictibacillus nanhaiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10.5 | the enzyme shows more than 50% of the maximal activity between pH 9.0 and 10.5 | Fictibacillus nanhaiensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | without any stabilizer, all samples except at pH 9.5, 10.0 and 6.0 maintain over 100% activity after being pre-treated in buffers ranging from pH 7.0-10.5 for 1 h at 30°C. The enzyme keeps more than 100% of its maximal activity after 1 h at 40°C (pH 7.0-9.0). Furthermore, it is stable under weak alkaline conditions (pH 7.0-9.0) after being pre-treated for 1 h at 50°C | Fictibacillus nanhaiensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Fictibacillus nanhaiensis |