Protein Variants | Comment | Organism |
---|---|---|
additional information | covalent immobilization of phenylalanine dehydrogenase onto commercially available enzyme carrier Eupergit CM for the synthesis of unnatural amino acid (S)-2-amino-4-phenylbutyric acid, method optimization and evaluation, overview. The immobilized enzyme shows increased thermostability, activity, broadened pH optimum, and remarkable improvement in operability and storage stability compared to the native enzyme. Immobilized PheDH is successfully applied for the synthesis of (S)-2-amino-4-phenylbutyric acid, achieving enantiomeric excess of more than 99% and yield of more than 80%; comparable to synthesis using the free PheDH | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | Rhodococcus sp. | - |
phenylpyruvate + NH3 + NADH + H+ | - |
r | |
L-phenylalanine + H2O + NAD+ | Rhodococcus sp. 4 | - |
phenylpyruvate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. 4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-phenylbutanoic acid + NH3 + NADH + H+ | - |
Rhodococcus sp. | (S)-2-amino-4-phenylbutyric acid + H2O + NAD+ | - |
r | |
2-oxo-4-phenylbutanoic acid + NH3 + NADH + H+ | - |
Rhodococcus sp. 4 | (S)-2-amino-4-phenylbutyric acid + H2O + NAD+ | - |
r | |
L-phenylalanine + H2O + NAD+ | - |
Rhodococcus sp. | phenylpyruvate + NH3 + NADH + H+ | - |
r | |
L-phenylalanine + H2O + NAD+ | - |
Rhodococcus sp. 4 | phenylpyruvate + NH3 + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
reductive amination, immobilized enzyme | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | reductive amination, immobilized enzyme | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodococcus sp. | |
NADH | - |
Rhodococcus sp. |