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Literature summary for 1.4.1.20 extracted from
- Purification of recombinant phenylalanine dehydrogenase by partitioning in aqueous two-phase systems (2007), J. Chromatogr. B, 854, 273-278.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) cells | Bacillus badius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
NAD+ | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Bacillus badius |  |
| 0.2 | - |
L-Phe | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Bacillus badius | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus badius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| in aqueous two-phase systems composed of polyethylene glycol 6000 and ammonium sulfate with 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and 13% (w/w) NaCl at pH 8.0 | Bacillus badius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the 474fold purified enzyme exhibits a specific activity of 10424.97 units/mg | Bacillus badius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-phenylalanine:NAD+ oxidoreductase, deaminating | - |
Bacillus badius |
| PheDH | - |
Bacillus badius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus badius | |
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Release 2023.1 (January 2023)
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