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Literature summary for 1.4.1.20 extracted from

  • Hanson, R.L.; Goldberg, S.L.; Brzozowski, D.B.; Tully, T.P.; Cazzulino, D.; Parker, W.L.; Lyngberg, O.K.; Vu, T.C.; Wong, M.K.; Patel, R.N.
    Preparation of an amino acid intermediate for the dipeptidyl peptidase IV inhibitor, saxagliptin, using a modified phenylalanine dehydrogenase (2007), Adv. Synth. Catal., 349, 1369-1378.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
the modified enzyme (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus) is expressed in Pichia pastoris strain SMD1168 or Escherichia coli strain SC 16496 Thermoactinomyces intermedius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 subunit of modified PDH (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus), SDS-PAGE Thermoactinomyces intermedius

Organism

Organism UniProt Comment Textmining
Thermoactinomyces intermedius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by Superdex 200 gel filtration Thermoactinomyces intermedius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + NH3 + NADH modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus is more effective with keto acid 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid and less effective with phenylpyruvate than the wild type PDH Thermoactinomyces intermedius (S)-3-hydroxyadamantylglycine + H2O + NAD+
-
 ?
phenylpyruvate + NH3 + NADH
-
 Thermoactinomyces intermedius L-Phe + H2O + NAD+
-
 r

Synonyms

Synonyms Comment Organism
PDH
-
 Thermoactinomyces intermedius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 wild type PDH retains most of its activity after 1 h at 60°C whereas modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus loses all activity after 1h at 60°C Thermoactinomyces intermedius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 9 modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus Thermoactinomyces intermedius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermoactinomyces intermedius