Cloned (Comment) | Organism |
---|---|
expression in Gdh-null Saccharomyces cerevisiae | Yarrowia lipolytica |
gene YALI0E09603g, cloned from strain INAG34815, sequence comparisons, recombinant expression in gdh-null Saccharomyces cerevisiae, recombinant overexpression in Yarrowia lipolytica strain under control of the constitutive TEF promoter using the JMP62 expression vectors. GDH activities in Yarrowia lipolytica are differentially expressed, overview | Yarrowia lipolytica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | YALI0E09603g gene deletion followed by growth on different carbon and nitrogen sources, and enzyme overexpression. Disruption of ylGDH1 and ylGDH2 (gdh1DELTA gdh2DELTA) completely abolishes both NADP- and NAD-GDH activities | Yarrowia lipolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Yarrowia lipolytica | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | Yarrowia lipolytica CLIB 122 | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | Q6C6H1 | - |
- |
Yarrowia lipolytica | Q6C6H1 | isoform GDH2 | - |
Yarrowia lipolytica CLIB 122 | Q6C6H1 | - |
- |
Yarrowia lipolytica CLIB 122 | Q6C6H1 | isoform GDH2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | - |
Yarrowia lipolytica | L-glutamate + H2O + NAD+ | - |
? | |
2-oxoglutarate + NH3 + NADH + H+ | - |
Yarrowia lipolytica CLIB 122 | L-glutamate + H2O + NAD+ | - |
? | |
L-glutamate + H2O + NAD+ | - |
Yarrowia lipolytica | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Yarrowia lipolytica CLIB 122 | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
c | - |
Yarrowia lipolytica |
NAD-dependent GDH | - |
Yarrowia lipolytica |
NAD-ylGdh2p | - |
Yarrowia lipolytica |
YALI0E09603g | - |
Yarrowia lipolytica |
ylGDH2 | - |
Yarrowia lipolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Yarrowia lipolytica | |
NADH | - |
Yarrowia lipolytica |
Organism | Comment | Expression |
---|---|---|
Yarrowia lipolytica | replacement of ammonia with glutamate in growth medium causes an increase in NAD-ylGdh2p activity | up |
General Information | Comment | Organism |
---|---|---|
metabolism | YALI0F17820g (ylGDH, EC 1.4.1.4) encodes a NADP-dependent GDH whereas YALI0E09603g (ylGDH2, EC 1.4.1.2) encodes a NAD-dependent GDH enzyme. The activity encoded by these two genes accounts for all measurable GDH activity in Yarrowia lipolytica. Levels of the two enzyme activities are comparable during logarithmic growth on rich medium, but the NADP-ylGDH1p enzyme activity is most highly expressed in stationary and nitrogen starved cells by 3fold to 12fold compared to NAD-ylGDH2p. Replacement of ammonia with glutamate causes a decrease in NADP-ylGdh1p activity, whereas NAD-ylGdh2p activity is increased. When glutamate is both carbon and nitrogen sources, the activity of NAD-ylGDH2p becomes dominant up to 18fold compared with that of NADP-ylGDH1p. ylGDH1 and ylGDH2 are functionally not interchangeable | Yarrowia lipolytica |
physiological function | gene YALI0F17820g (GDH1) encodes a NADP-dependent GDH whereas YALI0E09603g (GDH2) encodes a NAD-dependent GDH enzyme. The activity encoded by these two genes accounts for all measurable GDH activity in Yarrowia lipolytica. NAD-Gdh2 plays a role in nitrogen and carbon utilization from glutamate. GDH1 and GDH2 are not interchangeable | Yarrowia lipolytica |
physiological function | glutamate dehydrogenases (GDHs) are fundamental to cellular nitrogen and energy balance. NAD-ylGdh2p plays a role in nitrogen and carbon utilization from glutamate. Glutamate dehydrogenase (GDH) activity in gdh-null Saccharomyces cerevisiae mutant cells is restored by introduction of YALI0F17820g (ylGDH1, EC 1.4.1.4) or YALI0E09603g (ylGDH2) from Yarrowia lipolytica | Yarrowia lipolytica |