Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performs a dose-response curve with increase of 169% for NADH-dependent GDH with the [NH4+-N] increasing from 0 to 100 mg/l, while glutamine synthetase (GS) activity slightly changes. Compared to that, for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH records no major changes, while the GS increases slightly (17%) | Myriophyllum spicatum | |
NH4+ | the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performs a dose-response curve with increase of 169% for NADH-dependent GDH with the [NH4+-N] increasing from 0 to 100 mg/l, while glutamine synthetase (GS) activity slightly changes. Compared to that, for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH records no major changes, while the GS increases slightly (17%) | Potamogeton lucens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Myriophyllum spicatum | |
Ca2+ | required | Potamogeton lucens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Myriophyllum spicatum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | Potamogeton lucens | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myriophyllum spicatum | - |
- |
- |
Myriophyllum spicatum | - |
collected from Erhai Lake, a freshwater lake on the Yunnan-Guizhou Plateau, southwest China, ammonium-tolerant species | - |
Potamogeton lucens | - |
ammonium-sensitive species | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Myriophyllum spicatum | - |
leaf | - |
Potamogeton lucens | - |
shoot | - |
Myriophyllum spicatum | - |
shoot | - |
Potamogeton lucens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0495 | 0.133 | pH 8.2, 30°C | Myriophyllum spicatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Myriophyllum spicatum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Potamogeton lucens | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADH-dependent GDH | - |
Myriophyllum spicatum |
NADH-dependent GDH | - |
Potamogeton lucens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
reductive amination reaction, assay at | Myriophyllum spicatum |
30 | - |
reductive amination reaction, assay at | Potamogeton lucens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
reductive amination reaction, assay at | Myriophyllum spicatum |
8.2 | - |
reductive amination reaction, assay at | Potamogeton lucens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Myriophyllum spicatum | |
NAD+ | - |
Potamogeton lucens | |
NADH | - |
Myriophyllum spicatum | |
NADH | - |
Potamogeton lucens |
General Information | Comment | Organism |
---|---|---|
physiological function | in practical water restoration by aquatic plants, the alternative pathway of GDH is more important than the pathway catalyzed by GS in determining the tolerance of submerged macrophytes to high ammonium concentration. Both NADH-dependent and NADPH-dependent GDH (EC 1.4.1.4) show species-dependent variation, in the ammonium-tolerant species, Myriophyllum spicatum, there is a dose-response curve (from 49.46 to 132.99 nmol/min/mg protein for NADH-dependent GDH and 28.98 to 58.67 nmol/min/mg protein for NADPH-GDH), but in the ammonium-sensitive species, Potamogeton lucens, there is little change in activity | Myriophyllum spicatum |
physiological function | in practical water restoration by aquatic plants, the alternative pathway of GDH is more important than the pathway catalyzed by GS in determining the tolerance of submerged macrophytes to high ammonium concentration. Both NADH-dependent and NADPH-dependent GDH (EC 1.4.1.4) show species-dependent variation, in the ammonium-tolerant species, Myriophyllum spicatum, there is a dose-response curve (from 49.46 to 132.99 nmol/min/mg protein for NADH-dependent GDH and 28.98 to 58.67 nmol/min/mg protein for NADPH-GDH), but in the ammonium-sensitive species, Potamogeton lucens, there is little change in activity | Potamogeton lucens |
physiological function | the activity of glutamate dehydrogenase in the ammonium-tolerant species Myriophyllum spicatum leaves increases 169% for NADH-dependent GDH and 103% for NADPH-dependent GDH with the [NH4+-N] increasing from 0 to 100 mg/l, performing a dose-response curve while glutamine synthetase activity slightly changes | Myriophyllum spicatum |