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Literature summary for 1.4.1.2 extracted from

  • Kawakami, R.; Sakuraba, H.; Ohshima, T.
    Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis (2014), Biosci. Biotechnol. Biochem., 78, 2045-2050 .
    View publication on PubMed
Search for more literature for 1.4.1.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Janthinobacterium lividum

Protein Variants

Protein Variants Comment Organism
C1141T no large changes in catalytic activity Janthinobacterium lividum
C1141T residue C1141 is responsible for the inhibition of enzyme activity by HgCl2, and HgCl2 functions as an activating compound for mutant C1141T Janthinobacterium lividum
C825G no large changes in catalytic activity Janthinobacterium lividum
C872R no large changes in catalytic activity Janthinobacterium lividum
D869A no large changes in catalytic activity Janthinobacterium lividum
D885A complete loss of activtiy Janthinobacterium lividum
K810A complete loss of activtiy Janthinobacterium lividum
K820A complete loss of activtiy Janthinobacterium lividum
R784A complete loss of activtiy Janthinobacterium lividum
S1142A complete loss of activtiy Janthinobacterium lividum
V1139A no large changes in catalytic activity Janthinobacterium lividum

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, complete loss of activtiy for wild-type. 8fold increase in activity for mutant C1141T Janthinobacterium lividum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 NAD+ mutant C825G, pH 9.0, 25°C Janthinobacterium lividum
1.2
-
 NAD+ mutant V1139A, pH 9.0, 25°C Janthinobacterium lividum
1.3
-
 NAD+ wild-type, pH 9.0, 25°C Janthinobacterium lividum
1.3
-
 NAD+ mutant C872R, pH 9.0, 25°C Janthinobacterium lividum
1.5
-
 NAD+ mutant D869A, pH 9.0, 25°C Janthinobacterium lividum
2.1
-
 NAD+ mutant C1141T, pH 9.0, 25°C Janthinobacterium lividum
5.1
-
 L-glutamate mutant C872R, pH 9.0, 25°C Janthinobacterium lividum
5.2
-
 L-glutamate mutant C1141T, pH 9.0, 25°C Janthinobacterium lividum
5.4
-
 L-glutamate mutant C825G, pH 9.0, 25°C Janthinobacterium lividum
6.1
-
 L-glutamate wild-type, pH 9.0, 25°C Janthinobacterium lividum
6.8
-
 L-glutamate mutant D869A, pH 9.0, 25°C Janthinobacterium lividum
7.2
-
 L-glutamate mutant V1139A, pH 9.0, 25°C Janthinobacterium lividum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 1 mM, 2- and 2.8fold increase in activity, wild-type and mutant C1141T, respectively Janthinobacterium lividum

Organism

Organism UniProt Comment Textmining
Janthinobacterium lividum A5LH94
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Janthinobacterium lividum 2-oxoglutarate + NH3 + NADH + H+
-
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