Literature summary for 1.4.1.2 extracted from

Oliveira, T.; Sharkey, M.; Engel, P.; Khan, A.
Crystal structure of a chimaeric bacterial glutamate dehydrogenase (2016), Acta Crystallogr. Sect. F, 72, 462-466 .

Cloned(Commentary)

Cloned (Comment)	Organism
-	[Clostridium] symbiosum

Crystallization (Commentary)

Crystallization (Comment)	Organism
chimaeric protein consisting of domain I from NAD+-dependent GDH of Clostridium symbiosum, residues 1-200, domain II from NADP+-dependent GDH of Escherichia coli, residues 201-404 and the C-terminal helix again from Clostridium symbiosum, residues 405-448 which re-enters domain I. Domain II maintains its structural and functional integrity independently of the hinge and domain I. The enzyme is fully functional and retains the preference for NADP+ cofactor from the parent E. coli domain II	[Clostridium] symbiosum

Crystallization (Comment)

Organism

chimaeric protein consisting of domain I from NAD+-dependent GDH of Clostridium symbiosum, residues 1-200, domain II from NADP+-dependent GDH of Escherichia coli, residues 201-404 and the C-terminal helix again from Clostridium symbiosum, residues 405-448 which re-enters domain I. Domain II maintains its structural and functional integrity independently of the hinge and domain I. The enzyme is fully functional and retains the preference for NADP+ cofactor from the parent E. coli domain II

[Clostridium] symbiosum

Protein Variants

Protein Variants	Comment	Organism
additional information	chimaeric protein consisting of domain I from NAD+-dependent GDH of Clostridium symbiosum, residues 1-200, domain II from NADP+-dependent GDH of Escherichia coli, residues 201-404 and the C-terminal helix again from Clostridium symbiosum, residues 405-448 which re-enters domain I. Domain II maintains its structural and functional integrity independent of the hinge and domain I. The enzyme is fully functional and retains the preference for NADP+ cofactor from the parent E. coli domain II	[Clostridium] symbiosum

Organism

Organism	UniProt	Comment	Textmining
[Clostridium] symbiosum	P24295	-	-

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Release 2023.1 (January 2023)