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Literature summary for 1.4.1.2 extracted from

  • Fontaine, J.X.; Terce-Laforgue, T.; Bouton, S.; Pageau, K.; Lea, P.J.; Dubois, F.; Hirel, B.
    Further insights into the isoenzyme composition and activity of glutamate dehydrogenase in Arabidopsis thaliana (2013), Plant Signal. Behav., 8, e23329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes gdh1, gdh2 and gdh3, GDH1 gene encodes a polypeptide subunit termed beta and GDH2 gene encodes alpha and the polypeptides can be assembled as homo- or heterohexamers composed of different ratios of alpha and beta, leading to the formation of seven active isoenzymes Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Arabidopsis thaliana
-
2-oxoglutarate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
genes GDH1-3
-

Source Tissue

Source Tissue Comment Organism Textmining
companion cell
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information GDH3 is expressed only in the root companion cells, whereas the two others GDH1 and GDH2 are expressed in the same companion cells in both roots and shoots, tissue GDH isoenzyme composition, overview. The alpha, beta, and gamma polypeptides, that comprise the enzyme, can be assembled into a complex combination of heterohexamers in roots Arabidopsis thaliana
-
root three root isoenzymes Arabidopsis thaliana
-
stem floral Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Arabidopsis thaliana 2-oxoglutarate + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
heterohexamer the alpha, beta, and gamma polypeptides, that comprise the enzyme, can be assembled into a complex combination of heterohexamers in roots Arabidopsis thaliana
More GDH1 gene encodes a polypeptide subunit termed beta and GDH2 gene encodes alpha and the polypeptides can be assembled as homo- or heterohexamers composed of different ratios of alpha and beta, leading to the formation of seven active isoenzymes, complex pattern of isoenzymes, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GDH
-
Arabidopsis thaliana
NADH-dependent glutamate dehydrogenase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
additional information when NAD+ is replaced by NADP+, the GDH activity in the wild-type occurs only some specific homohexamer and heterohexamers with very low level Arabidopsis thaliana
NAD+
-
Arabidopsis thaliana
NADH highly preferred cofactor Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction when one or two of the three root isoenzymes are missing from the mutants, the remaining isoenzymes compensate for this deficiency Arabidopsis thaliana