Cloned (Comment) | Organism |
---|---|
genes gdh1, gdh2 and gdh3, GDH1 gene encodes a polypeptide subunit termed beta and GDH2 gene encodes alpha and the polypeptides can be assembled as homo- or heterohexamers composed of different ratios of alpha and beta, leading to the formation of seven active isoenzymes | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Arabidopsis thaliana | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
genes GDH1-3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
companion cell | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | GDH3 is expressed only in the root companion cells, whereas the two others GDH1 and GDH2 are expressed in the same companion cells in both roots and shoots, tissue GDH isoenzyme composition, overview. The alpha, beta, and gamma polypeptides, that comprise the enzyme, can be assembled into a complex combination of heterohexamers in roots | Arabidopsis thaliana | - |
root | three root isoenzymes | Arabidopsis thaliana | - |
stem | floral | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Arabidopsis thaliana | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | the alpha, beta, and gamma polypeptides, that comprise the enzyme, can be assembled into a complex combination of heterohexamers in roots | Arabidopsis thaliana |
More | GDH1 gene encodes a polypeptide subunit termed beta and GDH2 gene encodes alpha and the polypeptides can be assembled as homo- or heterohexamers composed of different ratios of alpha and beta, leading to the formation of seven active isoenzymes, complex pattern of isoenzymes, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Arabidopsis thaliana |
NADH-dependent glutamate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | when NAD+ is replaced by NADP+, the GDH activity in the wild-type occurs only some specific homohexamer and heterohexamers with very low level | Arabidopsis thaliana | |
NAD+ | - |
Arabidopsis thaliana | |
NADH | highly preferred cofactor | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | when one or two of the three root isoenzymes are missing from the mutants, the remaining isoenzymes compensate for this deficiency | Arabidopsis thaliana |