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Literature summary for 1.4.1.2 extracted from

  • Ferraro, G.; Bortolotti, S.; Mortera, P.; Schlereth, A.; Stitt, M.; Carrari, F.; Kamenetzky, L.; Valle, E.M.
    Novel glutamate dehydrogenase genes show increased transcript and protein abundances in mature tomato fruits (2012), J. Plant Physiol., 169, 899-907.
    View publication on PubMed
Search for more literature for 1.4.1.2

Cloned(Commentary)

Cloned (Comment) Organism
three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1, Slgdh-NAD;A1-3 show conserved structures, whereas Slgdh-NAD;B1 includes a 5'-untranslated exon, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expresssion analysis Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion four GDH deduced amino acid sequences possess the predicted mitochondrial target peptides, 18 amino acid residues at the N-terminus Solanum lycopersicum 5739
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Solanum lycopersicum
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 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+ Solanum lycopersicum Micro-Tom
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 2-oxoglutarate + NH3 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
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 three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1
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Solanum lycopersicum Micro-Tom
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 three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1
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Source Tissue

Source Tissue Comment Organism Textmining
fruit GDH activity increases during the fruit ripening along with the content of free glutamate, the most abundant amino acid of ripe fruit involved in conferring the genuine tomato flavour. Only the active homohexamer of GDH beta-subunits is detected in roots while heterohexamers of GDH alpha- and beta-subunits are found in fruits. alpha-Subunit Slgdh-NAD;A1-3 transcripts are detected in all tomato tissues examined, showing the highest levels in mature green fruits, contrasting with beta-subunit Slgdh-NAD;B1 transcripts which are detected mainly in roots or in mature fruits when treated with glutamate, NaCl or salicylic acid Solanum lycopersicum
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leaf
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 Solanum lycopersicum
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additional information subunit distributin and expression patterns in tissues of tomato, overview. The level of GDH alpha- and beta-subunits in tomato plants is regulated differently in each tomato organ Solanum lycopersicum
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root only the active homohexamer of GDH beta-subunits is detected in roots while heterohexamers of GDH alpha- and beta-subunits are found in fruits. alpha-Subunit Slgdh-NAD;A1-3 transcripts are detected in all tomato tissues examined, showing the highest levels in mature green fruits, contrasting with beta-subunit Slgdh-NAD;B1 transcripts which are detected mainly in roots or in mature fruits when treated with glutamate, NaCl or salicylic acid Solanum lycopersicum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Solanum lycopersicum 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+
-
 Solanum lycopersicum Micro-Tom 2-oxoglutarate + NH3 + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
NADH-GDH
-
 Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Solanum lycopersicum
NADH
-
 Solanum lycopersicum

General Information

General Information Comment Organism
additional information the level of GDH alpha- and beta-subunits in tomato plants is regulated differently in each tomato organ Solanum lycopersicum